Read Lab Publications

Located below is a list of the publications produced by our lab, starting with the most recent.


  • Ciganda M, Sotelo-Silveira J, Dubey AP, Pandey P, Smith JT, Shen S, Qu J, Smircich P, Read LK
  • Translational control by Trypanosoma brucei DRBD18 contributes to the maintenance of the procyclic state
  • RNA. 2023 Dec;29(12):1881-1895. doi: 10.1261/rna.079625.123. Epub 2023 Sep 20.
  • Smith JT, Tylec BL, Naguleswaran A, Roditi I, Read LK
  • Developmental dynamics of mitochondrial mRNA abundance and editing reveal roles for temperature and the differentiation-repressive kinase RDK1 in cytochrome oxidase subunit II mRNA editing
  • mBio. 2023 Oct 31;14(5):e0185423 doi: 10.1128/mbio.01854-23
  • Dubey AP, Tylec BL, Mishra A, Sortino K, Chen R, Sun Y, Read LK
  • KREH1 RNA helicase activity promotes utilization of initiator gRNAs across multiple mRNAs in trypanosome RNA editing
  • Nucleic Acids Res. 2023 Jun 23;51(11):5791-5809. doi: 10.1093/nar/gkad292


  • Sortino K, Tylec BL, Chen R, Sun Y, Read LK
  • Conserved and transcript-specific functions of the RESC factors, RESC13 and RESC14, in kinetoplastid RNA editing
  • RNA. 2022 Nov;28(11):1496-1508. doi: 10.1261/rna.079389.122. Epub 2022 Sep 12.


  • Mishra A, Kaur JN, McSkimming DI, Hegedusova E, Dubey AP, Ciganda M, Paris Z, Read LK
  • Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei
  • Mol Microbiol. 2021 Jun;116(3): 827-840. doi: 10.1111/mmi.14773
  • Dubey AP, Tylec BL, McAdams NM, Sortino K, Read LK
  • Trypanosome RNA Editing Substrate Binding Complex integrity and function depends on the upstream action of RESC10
  • Nucleic Acids Res. 2021 Apr;49(6):3557-3572. doi: 10.1093/nar/gkab129


  • Smith JT, Dolezelova E, Tylec BL, Bard JE, Chen R, Sun Y, Zikova A, Read LK
  • Developmental regulation of edited CYb and COIII mitochondrial mRNAs is achieved by distinct mechanisms in Trypanosoma brucei
  • Nucleic Acids Res. 2020 Sep;15(4):8704-8723. doi:
  • Aphasizheva I, ...21 authors..., Read L, and Aphasizhev R.
  • Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes
  • Trends Parasitol. 2020 Apr;36(4):337-355. doi: 10.1016/ Epub 2020 Feb 28.
  • Hashimoto H, Kafková L, Raczkowski A, Jordan KD, Read LK, Debler EW
  • Structural Basis of Protein Arginine Methyltransferase Activation by a Catalytically Dead Homolog (Prozyme)
  • J Mol Biol. 2020 Jan 17;432(2):410-426. doi: 10.1016/j.jmb.2019.11.002. Epub 2019 Nov 11


  • McAdams NM, Harrison GL, Tylec BL, Ammerman ML, Chen R, Sun Y, Read LK
  • MRB10130 is a RESC assembly factor that promotes kinetoplastid RNA editing initiation and progression
  • RNA. 2019 Sep;25(9):1177-1191. doi: 10.1261/rna.071902.119.
  • Tylec BL, Simpson RM, Kirby LE, Chen R, Sun Y, Koslowsky DJ, Read LK
  • Intrinsic and regulated properties of minimally edited trypanosome mRNAs
  • Nucleic Acids Res. 2019 Apr 23;47(7):3640-3657. doi: 10.1093/nar/gkz012.


  • Zimmer SL, Simpson RM, Read LK
  • High throughput sequencing revolution reveals conserved fundamentals of U-indel editing
  • WIREs RNA. 2018;9:e1487


  • Simpson RM, Bruno AE, Chen R, Lott K, Tylec BL, Bard JE, Sun Y, Buck MJ, Read LK
  • Trypanosome RNA Editing Mediator Complex proteins have distinct functions in gRNA utilization
  • Nucleic Acids Research. 2017 Jul 27;45(13):7965-7983. doi: 10.1093/nar/gkx458.
  • Kafková L, Debler EW, Fisk JC, Jain K, Clarke SG, Read LK
  • The Major Protein Arginine Methyltransferase in Trypanosoma brucei Functions as an Enzyme-Prozyme Complex
  • J Biol Chem. 2017 Feb 10;292(6):2089-2100. doi: 10.1074/jbc.M116.757112. Epub 2016 Dec 20.


  • Kumar V, Madina BR, Gulati S, Vashisht AA, Kanyumbu C, Pieters B, Shakir A, Wohlschlegel JA, Read LK, Mooers BH, Cruz-Reyes J.
  • REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes.
  • J Biol Chem. 2016 Mar 11;291(11):5753-64. doi: 10.1074/jbc.M115.708164. Epub 2016 Jan 14.
  • Read LK, Lukeš J, Hashimi H.
  • Trypanosome RNA editing: the complexity of getting U in and taking U out.
  • Wiley Interdiscip Rev RNA. 2016 Jan;7(1):33-51.


  • Shaw PL, McAdams NM, Hast MA, Ammerman ML, Read LK, Schumacher MA.
  • Structures of the T.brucei kRNA editing factor MRB1590 reveal unique RNA-binding pore motif contained within an ABC-ATPase fold.
  • Nucleic Acids Res. 2015 Aug 18;43(14):7096-109
  • Lott K, Mukhopadhyay S, Li J, Wang J, Yao J, Sun Y, Qu J, Read LK.
  • Arginine methylation of DRBD18 differentially impacts its opposing effects on the trypanosome transcriptome.
  • Nucleic Acids Res. 2015 Jun 23;43(11):5501-23.
  • McAdams NM., Ammerman ML., Nanduri J., Lott K., Fisk JC., Read LK.
  • An arginine-glycine rich RNA binding protein impacts the abundance of specific mRNAs in the mitochondria of Trypanosoma brucei.
  • Eukaryot Cell. 2014 Dec 5. pii: EC.00232-14. [Epub ahead of print]


  • Lott K., Zhu L., Fisk J., Tomasello DL., Read LK.
  • Functional interplay between protein arginine methyltransferases in Trypanosoma brucei.
  • Microbiology Open 3(5):595-609.


  • Ammerman ML, Tomasello DL, Faktorová D, Kafková L, Hashimi H, Lukes J, Read LK.
  • A core MRB1 complex component is indispensable for RNA editing in insect and human infective stages of Trypanosoma brucei.
  • PLoS One. 2013 Oct 18;8(10):e78015. doi: 10.1371/journal.pone.0078015.
  • Sakyiama J, Zimmer SL, Ciganda M, Williams N, Read LK.
  • Ribosome biogenesis requires a highly diverged XRN family 5' ? 3' exoribonuclease for rRNA processing in Trypanosoma brucei.
  • RNA. 2013 Oct;19(10):1419-31. doi: 10.1261/rna.038547.113. Epub 2013 Aug 23.
  • Lott K, Li J, Fisk JC, Wang H, Aletta JM, Qu J, Read LK.
  • Global proteomic analysis in trypanosomes reveals unique proteins and conserved cellular processes impacted by arginine methylation.
  • J Proteomics. 2013 Jul 19;91C:210-225. doi: 10.1016/j.jprot.2013.07.010. [Epub ahead of print]
  • Hashimi H, Zimmer SL, Ammerman ML, Read LK, Lukes J.
  • Dual core processing: MRB1 is an emerging kinetoplast RNA editing complex.
  • Trends Parasitol. 2013 Feb;29(2):91-9. doi: 10.1016/ Epub 2013 Jan 8. Review.
  • Fisk JC, Li J, Wang H, Aletta JM, Qu J, Read LK.
  • Proteomic analysis reveals diverse classes of arginine methylproteins in mitochondria of trypanosomes.
  • Mol Cell Proteomics. 2013 Feb;12(2):302-11. doi: 10.1074/mcp.M112.022533. Epub 2012 Nov 14.


  • Kafková L, Ammerman ML, Faktorová D, Fisk JC, Zimmer SL, Sobotka R, Read LK, Lukes J, Hashimi H.
  • Functional characterization of two paralogs that are novel RNA binding proteins influencing mitochondrial transcripts of Trypanosoma brucei.
  • RNA. 18(10):1846-61
  • Foda BM, Downey KM, Fisk JC, Read LK.
  • Multifunctional G-Rich and RRM-Containing Domains of TbRGG2 Perform Separate yet Essential Functions in Trypanosome RNA Editing.
  • Eukaryot Cell. 11(9):1119-31.
  • Zimmer SL, McEvoy SM, Menon S, Read LK
  • Additive and Transcript-Specific Effects of KPAP1 and TbRND Activities on 3' Non-Encoded Tail Characteristics and mRNA Stability in Trypanosoma brucei.
  • PLoS One. 7(5):e37639
  • Ammerman ML, Downey KM, Hashimi H, Fisk JC, Tomasello DL, Faktorová D, Kafková L, King T, Lukes J, Read LK
  • Architecture of the trypanosome RNA editing accessory complex, MRB1.
  • Nucleic Acids Res. 40(12):5637-50


  • Madina BR, Kuppan G, Vashisht AA, Liang YH, Downey KM, Wohlschlegel JA, Ji X, Sze SH, Sacchettini JC, Read LK, Cruz-Reyes J.
  • Guide RNA biogenesis involves a novel RNase III family endoribonuclease in Trypanosoma brucei.
  • RNA. 17(10):1821-3
  • Fisk JC, Read LK.
  • Protein arginine methylation in parasitic protozoa.
  • Eukaryot Cell. 10(8):1013-22
  • Ammerman ML, Hashimi H, Novotná L, Cicová Z, McEvoy SM, Lukes J, Read LK.
  • MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process.
  • RNA 17(5):865-77
  • Read LK, Zimmer SL, Ammerman ML.
  • Marked for translation: long A/U tails as an interface between completion of RNA editing and ribosome recruitment.
  • Mol Cell 42(1):106-17
  • Zimmer SL, McEvoy SM, Li J, Qu J, Read LK.
  • A novel member of the RNase D exoribonuclease family functions in mitochondrial guide RNA metabolism in Trypanosoma brucei.
  • J Biol Chem. 286(12):10329-340


  • Fisk JC, Zurita-Lopez C, Sayegh J, Tomasello DL, Clarke SG, Read LK.
  • TbPRMT6 is a type I protein arginine methyltransferase that contributes to cytokinesis in Trypanosoma brucei.
  • Eukaryot Cell. 9(6):866-77
  • Sprehe M, Fisk JC, McEvoy SM, Read LK, Schumacher MA.
  • Structure of the T. brucei p22 protein, a cytochrome oxidase subunit II (COII) specific RNA editing accessory factor.
  • J Biol Chem. 285(24):18899-908


  • Fisk JC, Presnyak V, Ammerman ML, Read LK.
  • Distinct and overlapping functions of MRP1/2 and RBP16 in mitochondrial RNA metabolism.
  • Mol Cell Biol. 29(19):5214-25
  • Mattiacio JL, Read LK.
  • Evidence for a degradosome-like complex in the mitochondria of Trypanosoma brucei.
  • FEBS Lett. 583(14):2333-8
  • Fisk JC, Sayegh J, Zurita-Lopez C, Menon S, Presnyak V, Clarke SG, Read LK.
  • A type III protein arginine methyltransferase from the protozoan parasite Trypanosoma brucei.
  • J Biol Chem. 284(17):11590-600


  • Fisk JC, Ammerman ML, Presnyak V, Read LK.
  • TbRGG2, an essential RNA editing accessory factor in two Trypanosoma brucei life cycle stages.
  • J Biol Chem. 283(34):23016-25
  • Ammerman ML, Fisk JC, Read LK.
  • gRNA/pre-mRNA annealing and RNA chaperone activities of RBP16.
  • RNA. 14(6):1069-80
  • Mattiacio JL, Read LK.
  • Roles for TbDSS-1 in RNA surveillance and decay of maturation by-products from the 12S rRNA locus.
  • Nucleic Acids Res. 36(1):319-29


  • Pelletier M, Read LK, Aphasizhev R.
  • Isolation of RNA binding proteins involved in insertion/deletion editing.
  • Methods Enzymol. 424:75-105
  • Pusnik M, Small I, Read LK, Fabbro T, Schneider A.
  • Pentatricopeptide repeat proteins in Trypanosoma brucei function in mitochondrial ribosomes.
  • Mol Cell Biol. 27(19):6876-88
  • Pasternack DA, Sayegh J, Clarke S, Read LK.
  • Evolutionarily divergent type II protein arginine methyltransferase in Trypanosoma brucei.
  • Eukaryot Cell. 6(9):1665-81
  • Kao CY, Read LK.
  • Targeted depletion of a mitochondrial nucleotidyltransferase suggests the presence of multiple enzymes that polymerize mRNA 3' tails in Trypanosoma brucei mitochondria.
  • Mol Biochem Parasitol. 154(2):158-69
  • Duan P, Xu Y, Birkaya B, Myers J, Pelletier M, Read LK, Guarnaccia C, Pongor S, Denman RB, Aletta JM.
  • Generation of polyclonal antiserum for the detection of methylarginine proteins.
  • J Immunol Methods. 320(1-2):132-42
  • Goulah CC, Read LK.
  • Differential effects of arginine methylation on RBP16 mRNA binding, guide RNA (gRNA) binding, and gRNA-containing ribonucleoprotein complex (gRNP) formation.
  • J Biol Chem. 282(10):7181-90


  • Goulah CC, Pelletier M, Read LK.
  • Arginine methylation regulates mitochondrial gene expression in Trypanosoma brucei through multiple effector proteins.
  • RNA. 12(8):1545-55
  • Miller MM, Halbig K, Cruz-Reyes J, Read LK
  • RBP16 stimulates trypanosome RNA editing in vitro at an early step in the editing reaction.
  • RNA. 12(7):1292-303
  • Ryan CM, Kao CY, Sleve DA, Read LK.
  • Biphasic decay of guide RNAs in Trypanosoma brucei.
  • Mol Biochem Parasitol. 146(1):68-77


  • Pelletier M, Pasternack DA, Read LK.
  • In vitro and in vivo analysis of the major type I protein arginine methyltransferase from Trypanosoma brucei.
  • Mol Biochem Parasitol. 144(2):206-17
  • Ryan CM, Read LK.
  • UTP-dependent turnover of Trypanosoma brucei mitochondrial mRNA requires UTP polymerization and involves the RET1 TUTase.
  • RNA. 11(5):763-73
  • Kao CY, Read LK.
  • Opposing effects of polyadenylation on the stability of edited and unedited mitochondrial RNAs in Trypanosoma brucei.
  • Mol Cell Biol. 25(5):1634-44


  • Penschow JL, Sleve DA, Ryan CM, Read LK.
  • TbDSS-1, an essential Trypanosoma brucei exoribonuclease homolog that has pleiotropic effects on mitochondrial RNA metabolism.
  • Eukaryot Cell. 3(5):1206-16


  • Miller MM, Read LK.
  • Trypanosoma brucei: functions of RBP16 cold shock and RGG domains in macromolecular interactions.
  • Exp Parasitol. 105(2):140-8
  • Ryan CM, Militello KT, Read LK.
  • Polyadenylation regulates the stability of Trypanosoma brucei mitochondrial RNAs.
  • J Biol Chem. 278(35):32753-62
  • Pelletier M, Read LK.
  • RBP16 is a multifunctional gene regulatory protein involved in editing and stabilization of specific mitochondrial mRNAs in Trypanosoma brucei.
  • RNA. 9(4):457-68




  • Hayman, M.L., M.M. Miller, D.M. Chandler, C.C. Goulah, and L.K. Read.
  • The trypanosome homolog of human p32 interacts with RBP16 and stimulates its gRNA binding activity.
  • Nucl. Acids Res. 29(24):5216-5225
  • Pelletier, M., Y. Xu, X. Wang, S. Zahariev, S. Pongor, J.M. Aletta, and L.K. Read.
  • Arginine methylation of a mitochondrial guide RNA binding protein from Trypanosoma brucei.
  • Mol. Biochem. Parasitol. 118(1):49-59


  • Militello, K.T., M.L. Hayman, and L.K. Read.
  • Transcriptional and post-transcriptional in organello labeling of Trypanosoma brucei mitochondrial RNA.
  • Int. J. Parasitol. 30:643-647
  • Militello, K.T. and L.K. Read.
  • UTP-dependent and UTP-independent pathways of mRNA turnover in Trypanosoma brucei mitochondria.
  • Mol. Cell. Biol. 20(7):2308-2316
  • Pelletier, M., M.M. Miller, and L.K. Read.
  • RNA binding properties of the mitochondrial Y-box protein RBP16.
  • Nucl. Acids Res. 28(5):1266-1275


  • Read, L.K., K.T. Militello, and G.E. Nerantzakis.
  • Cloning and characterisation of a cDNA encoding the Trypanosoma brucei ribosomal protein L24.
  • Int. J. Parasitol. 29(4):601-605
  • Hayman, M.L. and L.K. Read.
  • Trypanosoma brucei RBP16 is a mitochondrial Y-box family protein with guide RNA binding activity.
  • J. Biol. Chem. 274(17):12067-12074
  • Militello, K.T. and L.K. Read.
  • Coordination of kRNA editing and polyadenylation in Trypanosoma brucei mitochondria: Complete editing is not required for long poly(A) tract addition.
  • Nucl. Acids Res. 27(5):1377-1385
  • Hotchkiss, T.L., G.E. Nerantzakis, S.C. Dills, L. Shang, and L.K. Read.
  • Trypanosoma brucei poly(A) binding protein I cloning, expression, and binding to 5' untranslated region sequence elements.
  • Mol. Biochem. Parasitol. 98(1):117-129