The Read Lab

2023

• Ciganda M, Sotelo-Silveira J, Dubey AP, Pandey P, Smith JT, Shen S, Qu J, Smircich P, Read LK
• Translational control by Trypanosoma brucei DRBD18 contributes to the maintenance of the procyclic state
• RNA. 2023 Dec;29(12):1881-1895. doi: 10.1261/rna.079625.123. Epub 2023 Sep 20.
• Abstract PDF Suppl. Data

• Smith JT, Tylec BL, Naguleswaran A, Roditi I, Read LK
• Developmental dynamics of mitochondrial mRNA abundance and editing reveal roles for temperature and the differentiation-repressive kinase RDK1 in cytochrome oxidase subunit II mRNA editing
• mBio. 2023 Oct 31;14(5):e0185423 doi: 10.1128/mbio.01854-23
• Abstract PDF Suppl. Data

• Dubey AP, Tylec BL, Mishra A, Sortino K, Chen R, Sun Y, Read LK
• KREH1 RNA helicase activity promotes utilization of initiator gRNAs across multiple mRNAs in trypanosome RNA editing
• Nucleic Acids Res. 2023 Jun 23;51(11):5791-5809. doi: 10.1093/nar/gkad292
• Abstract PDF Suppl. Data

2022

• Sortino K, Tylec BL, Chen R, Sun Y, Read LK
• Conserved and transcript-specific functions of the RESC factors, RESC13 and RESC14, in kinetoplastid RNA editing
• RNA. 2022 Nov;28(11):1496-1508. doi: 10.1261/rna.079389.122. Epub 2022 Sep 12.
• Abstract PDF Suppl. Data

2021

• Mishra A, Kaur JN, McSkimming DI, Hegedusova E, Dubey AP, Ciganda M, Paris Z, Read LK
• Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei
• Mol Microbiol. 2021 Jun;116(3): 827-840. doi: 10.1111/mmi.14773
• Abstract PDF Suppl. Data

• Dubey AP, Tylec BL, McAdams NM, Sortino K, Read LK
• Trypanosome RNA Editing Substrate Binding Complex integrity and function depends on the upstream action of RESC10
• Nucleic Acids Res. 2021 Apr;49(6):3557-3572. doi: 10.1093/nar/gkab129
• Abstract PDF Suppl. Data

2020

• Smith JT, Dolezelova E, Tylec BL, Bard JE, Chen R, Sun Y, Zikova A, Read LK
• Developmental regulation of edited CYb and COIII mitochondrial mRNAs is achieved by distinct mechanisms in Trypanosoma brucei
• Nucleic Acids Res. 2020 Sep;15(4):8704-8723. doi: https://doi.org/10.1093/nar/gkaa641
• Abstract PDF Suppl. Data

• Aphasizheva I, ...21 authors..., Read L, and Aphasizhev R.
• Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes
• Trends Parasitol. 2020 Apr;36(4):337-355. doi: 10.1016/j.pt.2020.01.006. Epub 2020 Feb 28.
• Abstract PDF

• Hashimoto H, Kafková L, Raczkowski A, Jordan KD, Read LK, Debler EW
• Structural Basis of Protein Arginine Methyltransferase Activation by a Catalytically Dead Homolog (Prozyme)
• J Mol Biol. 2020 Jan 17;432(2):410-426. doi: 10.1016/j.jmb.2019.11.002. Epub 2019 Nov 11
• Abstract PDF Suppl. Data

2019

• McAdams NM, Harrison GL, Tylec BL, Ammerman ML, Chen R, Sun Y, Read LK
• MRB10130 is a RESC assembly factor that promotes kinetoplastid RNA editing initiation and progression
• RNA. 2019 Sep;25(9):1177-1191. doi: 10.1261/rna.071902.119.
• Abstract PDF Suppl. Tables 1-5

• Tylec BL, Simpson RM, Kirby LE, Chen R, Sun Y, Koslowsky DJ, Read LK
• Intrinsic and regulated properties of minimally edited trypanosome mRNAs
• Nucleic Acids Res. 2019 Apr 23;47(7):3640-3657. doi: 10.1093/nar/gkz012.
• Abstract PDF Supplementary data Suppl. Tables 1-4

2018

• Kafková L, Tu C, Pazzo KL, Smith KP, Debler EW, Paul KS, Qu J, Read LK
• Trypanosoma brucei PRMT1 is a nucleic acid binding protein with a role in energy metabolism and the starvation stress response
• mBio 9:e02430-18
• Abstract PDF Fig. S1 Suppl. Table 1 Suppl. Table 2 Suppl. Text 1 Suppl. Table 3 Suppl. Table 4

• Zimmer SL, Simpson RM, Read LK
• High throughput sequencing revolution reveals conserved fundamentals of U-indel editing
• WIREs RNA. 2018;9:e1487
• Abstract PDF

• McAdams NM, Simpson RM, Chen R, Sun Y, Read L
• MRB7260 is essential for productive protein-RNA interactions within the RNA editing substrate binding complex during trypanosome RNA editing
• RNA 24:540-556
• Abstract PDF Fig. S1 Fig. S2 Fig. S3 Fig. S4 Fig. S5 Fig. S6 Suppl. Table 1 Suppl. Table 2 Suppl. Table 3 Suppl. Legends Suppl. Methods

2017

• Simpson RM, Bruno AE, Chen R, Lott K, Tylec BL, Bard JE, Sun Y, Buck MJ, Read LK
• Trypanosome RNA Editing Mediator Complex proteins have distinct functions in gRNA utilization
• Nucleic Acids Research. 2017 Jul 27;45(13):7965-7983. doi: 10.1093/nar/gkx458.
• Abstract PDF supplemental 1 supplemental 2

• Kafková L, Debler EW, Fisk JC, Jain K, Clarke SG, Read LK
• The Major Protein Arginine Methyltransferase in Trypanosoma brucei Functions as an Enzyme-Prozyme Complex
• J Biol Chem. 2017 Feb 10;292(6):2089-2100. doi: 10.1074/jbc.M116.757112. Epub 2016 Dec 20.
• Abstract PDF

2016

• Simpson RM, Bruno AE, Bard JE, Buck MJ, Read LK.
• High-throughput sequencing of partially edited trypanosome mRNAs reveals barriers to editing progression and evidence for alternative editing.
• RNA. 2016 May;22(5):677-95. doi: 10.1261/rna.055160.115. Epub 2016 Feb 23.
• Abstract PDF Supp Figure S1.tif Supp Figure S2.tif Supp Figure S3.tif Supp Figure S4.tif Supp Legends Supp Tables

• Kumar V, Madina BR, Gulati S, Vashisht AA, Kanyumbu C, Pieters B, Shakir A, Wohlschlegel JA, Read LK, Mooers BH, Cruz-Reyes J.
• REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes.
• J Biol Chem. 2016 Mar 11;291(11):5753-64. doi: 10.1074/jbc.M115.708164. Epub 2016 Jan 14.
• Abstract PDF

• Read LK, Lukeš J, Hashimi H.
• Trypanosome RNA editing: the complexity of getting U in and taking U out.
• Wiley Interdiscip Rev RNA. 2016 Jan;7(1):33-51.
• Abstract PDF

2015

• Huang Z, Faktorová D, Krízová A, Kafková L, Read LK, Lukeš J, Hashimi H.
• Integrity of the core mitochondrial RNA-binding complex 1 is vital for trypanosome RNA editing.
• RNA. 2015 Dec;21(12):2088-102.
• Abstract PDF supplemental 1 supplemental 2 supplemental 3

• Shaw PL, McAdams NM, Hast MA, Ammerman ML, Read LK, Schumacher MA.
• Structures of the T.brucei kRNA editing factor MRB1590 reveal unique RNA-binding pore motif contained within an ABC-ATPase fold.
• Nucleic Acids Res. 2015 Aug 18;43(14):7096-109
• Abstract PDF supplemental 1

• Lott K, Mukhopadhyay S, Li J, Wang J, Yao J, Sun Y, Qu J, Read LK.
• Arginine methylation of DRBD18 differentially impacts its opposing effects on the trypanosome transcriptome.
• Nucleic Acids Res. 2015 Jun 23;43(11):5501-23.
• Abstract PDF supplemental 1 supplemental 2 supplemental 3

• McAdams NM., Ammerman ML., Nanduri J., Lott K., Fisk JC., Read LK.
• An arginine-glycine rich RNA binding protein impacts the abundance of specific mRNAs in the mitochondria of Trypanosoma brucei.
• Eukaryot Cell. 2014 Dec 5. pii: EC.00232-14. [Epub ahead of print]
• Abstract PDF

2014

• Lott K., Zhu L., Fisk J., Tomasello DL., Read LK.
• Functional interplay between protein arginine methyltransferases in Trypanosoma brucei.
• Microbiology Open 3(5):595-609.
• Abstract PDF

2013

• Ammerman ML, Tomasello DL, Faktorová D, Kafková L, Hashimi H, Lukes J, Read LK.
• A core MRB1 complex component is indispensable for RNA editing in insect and human infective stages of Trypanosoma brucei.
• PLoS One. 2013 Oct 18;8(10):e78015. doi: 10.1371/journal.pone.0078015.
• Abstract PDF

• Sakyiama J, Zimmer SL, Ciganda M, Williams N, Read LK.
• Ribosome biogenesis requires a highly diverged XRN family 5' ? 3' exoribonuclease for rRNA processing in Trypanosoma brucei.
• RNA. 2013 Oct;19(10):1419-31. doi: 10.1261/rna.038547.113. Epub 2013 Aug 23.
• Abstract PDF supplemental 1 supplemental 2

• Lott K, Li J, Fisk JC, Wang H, Aletta JM, Qu J, Read LK.
• Global proteomic analysis in trypanosomes reveals unique proteins and conserved cellular processes impacted by arginine methylation.
• J Proteomics. 2013 Jul 19;91C:210-225. doi: 10.1016/j.jprot.2013.07.010. [Epub ahead of print]
• Abstract PDF supplemental 1 supplemental 2

• Hashimi H, Zimmer SL, Ammerman ML, Read LK, Lukes J.
• Dual core processing: MRB1 is an emerging kinetoplast RNA editing complex.
• Trends Parasitol. 2013 Feb;29(2):91-9. doi: 10.1016/j.pt.2012.11.005. Epub 2013 Jan 8. Review.
• Abstract PDF

• Fisk JC, Li J, Wang H, Aletta JM, Qu J, Read LK.
• Proteomic analysis reveals diverse classes of arginine methylproteins in mitochondria of trypanosomes.
• Mol Cell Proteomics. 2013 Feb;12(2):302-11. doi: 10.1074/mcp.M112.022533. Epub 2012 Nov 14.
• Abstract PDF supplemental 1 supplemental 2

2012

• Kafková L, Ammerman ML, Faktorová D, Fisk JC, Zimmer SL, Sobotka R, Read LK, Lukes J, Hashimi H.
• Functional characterization of two paralogs that are novel RNA binding proteins influencing mitochondrial transcripts of Trypanosoma brucei.
• RNA. 18(10):1846-61
• Abstract PDF supplemental

• Foda BM, Downey KM, Fisk JC, Read LK.
• Multifunctional G-Rich and RRM-Containing Domains of TbRGG2 Perform Separate yet Essential Functions in Trypanosome RNA Editing.
• Eukaryot Cell. 11(9):1119-31.
• Abstract PDF supplemental

• Zimmer SL, McEvoy SM, Menon S, Read LK
• Additive and Transcript-Specific Effects of KPAP1 and TbRND Activities on 3' Non-Encoded Tail Characteristics and mRNA Stability in Trypanosoma brucei.
• PLoS One. 7(5):e37639
• Abstract PDF supplemental 1 supplemental 2

• Ammerman ML, Downey KM, Hashimi H, Fisk JC, Tomasello DL, Faktorová D, Kafková L, King T, Lukes J, Read LK
• Architecture of the trypanosome RNA editing accessory complex, MRB1.
• Nucleic Acids Res. 40(12):5637-50
• Abstract PDF supplemental

2011

• Madina BR, Kuppan G, Vashisht AA, Liang YH, Downey KM, Wohlschlegel JA, Ji X, Sze SH, Sacchettini JC, Read LK, Cruz-Reyes J.
• Guide RNA biogenesis involves a novel RNase III family endoribonuclease in Trypanosoma brucei.
• RNA. 17(10):1821-3
• Abstract PDF supplemental 1 supplemental 2 supplemental 3

• Fisk JC, Read LK.
• Protein arginine methylation in parasitic protozoa.
• Eukaryot Cell. 10(8):1013-22
• Abstract PDF

• Ammerman ML, Hashimi H, Novotná L, Cicová Z, McEvoy SM, Lukes J, Read LK.
• MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process.
• RNA 17(5):865-77
• Abstract PDF supplemental 1 supplemental 2

• Read LK, Zimmer SL, Ammerman ML.
• Marked for translation: long A/U tails as an interface between completion of RNA editing and ribosome recruitment.
• Mol Cell 42(1):106-17
• Abstract PDF

• Zimmer SL, McEvoy SM, Li J, Qu J, Read LK.
• A novel member of the RNase D exoribonuclease family functions in mitochondrial guide RNA metabolism in Trypanosoma brucei.
• J Biol Chem. 286(12):10329-340
• Abstract PDF supplemental

2010

• Ammerman ML, Presnyak V, Fisk JC, Foda BM, Read LK.
• TbRGG2 facilitates kinetoplastid RNA editing initiation and progression past intrinsic pause sites.
• RNA. 16:2239-2251
• Abstract PDF supplemental 1 supplemental 2 supplemental 3 supplemental 4

• Fisk JC, Zurita-Lopez C, Sayegh J, Tomasello DL, Clarke SG, Read LK.
• TbPRMT6 is a type I protein arginine methyltransferase that contributes to cytokinesis in Trypanosoma brucei.
• Eukaryot Cell. 9(6):866-77
• Abstract PDF supplemental

• Sprehe M, Fisk JC, McEvoy SM, Read LK, Schumacher MA.
• Structure of the T. brucei p22 protein, a cytochrome oxidase subunit II (COII) specific RNA editing accessory factor.
• J Biol Chem. 285(24):18899-908
• Abstract PDF supplemental

2009

• Fisk JC, Presnyak V, Ammerman ML, Read LK.
• Distinct and overlapping functions of MRP1/2 and RBP16 in mitochondrial RNA metabolism.
• Mol Cell Biol. 29(19):5214-25
• Abstract PDF

• Mattiacio JL, Read LK.
• Evidence for a degradosome-like complex in the mitochondria of Trypanosoma brucei.
• FEBS Lett. 583(14):2333-8
• Abstract PDF

• Fisk JC, Sayegh J, Zurita-Lopez C, Menon S, Presnyak V, Clarke SG, Read LK.
• A type III protein arginine methyltransferase from the protozoan parasite Trypanosoma brucei.
• J Biol Chem. 284(17):11590-600
• Abstract PDF

2008

• Fisk JC, Ammerman ML, Presnyak V, Read LK.
• TbRGG2, an essential RNA editing accessory factor in two Trypanosoma brucei life cycle stages.
• J Biol Chem. 283(34):23016-25
• Abstract PDF

• Ammerman ML, Fisk JC, Read LK.
• gRNA/pre-mRNA annealing and RNA chaperone activities of RBP16.
• RNA. 14(6):1069-80
• Abstract PDF

• Mattiacio JL, Read LK.
• Roles for TbDSS-1 in RNA surveillance and decay of maturation by-products from the 12S rRNA locus.
• Nucleic Acids Res. 36(1):319-29
• Abstract PDF

2007

• Pelletier M, Read LK, Aphasizhev R.
• Isolation of RNA binding proteins involved in insertion/deletion editing.
• Methods Enzymol. 424:75-105
• Abstract PDF

• Pusnik M, Small I, Read LK, Fabbro T, Schneider A.
• Pentatricopeptide repeat proteins in Trypanosoma brucei function in mitochondrial ribosomes.
• Mol Cell Biol. 27(19):6876-88
• Abstract PDF

• Pasternack DA, Sayegh J, Clarke S, Read LK.
• Evolutionarily divergent type II protein arginine methyltransferase in Trypanosoma brucei.
• Eukaryot Cell. 6(9):1665-81
• Abstract PDF

• Kao CY, Read LK.
• Targeted depletion of a mitochondrial nucleotidyltransferase suggests the presence of multiple enzymes that polymerize mRNA 3' tails in Trypanosoma brucei mitochondria.
• Mol Biochem Parasitol. 154(2):158-69
• Abstract PDF

• Duan P, Xu Y, Birkaya B, Myers J, Pelletier M, Read LK, Guarnaccia C, Pongor S, Denman RB, Aletta JM.
• Generation of polyclonal antiserum for the detection of methylarginine proteins.
• J Immunol Methods. 320(1-2):132-42
• Abstract PDF

• Goulah CC, Read LK.
• Differential effects of arginine methylation on RBP16 mRNA binding, guide RNA (gRNA) binding, and gRNA-containing ribonucleoprotein complex (gRNP) formation.
• J Biol Chem. 282(10):7181-90
• Abstract PDF

2006

• Goulah CC, Pelletier M, Read LK.
• Arginine methylation regulates mitochondrial gene expression in Trypanosoma brucei through multiple effector proteins.
• RNA. 12(8):1545-55
• Abstract PDF

• Miller MM, Halbig K, Cruz-Reyes J, Read LK
• RBP16 stimulates trypanosome RNA editing in vitro at an early step in the editing reaction.
• RNA. 12(7):1292-303
• Abstract PDF

• Ryan CM, Kao CY, Sleve DA, Read LK.
• Biphasic decay of guide RNAs in Trypanosoma brucei.
• Mol Biochem Parasitol. 146(1):68-77
• Abstract PDF

2005

• Pelletier M, Pasternack DA, Read LK.
• In vitro and in vivo analysis of the major type I protein arginine methyltransferase from Trypanosoma brucei.
• Mol Biochem Parasitol. 144(2):206-17
• Abstract PDF

• Ryan CM, Read LK.
• UTP-dependent turnover of Trypanosoma brucei mitochondrial mRNA requires UTP polymerization and involves the RET1 TUTase.
• RNA. 11(5):763-73
• Abstract PDF

• Kao CY, Read LK.
• Opposing effects of polyadenylation on the stability of edited and unedited mitochondrial RNAs in Trypanosoma brucei.
• Mol Cell Biol. 25(5):1634-44
• Abstract PDF

2004

• Penschow JL, Sleve DA, Ryan CM, Read LK.
• TbDSS-1, an essential Trypanosoma brucei exoribonuclease homolog that has pleiotropic effects on mitochondrial RNA metabolism.
• Eukaryot Cell. 3(5):1206-16
• Abstract PDF

2003

• Miller MM, Read LK.
• Trypanosoma brucei: functions of RBP16 cold shock and RGG domains in macromolecular interactions.
• Exp Parasitol. 105(2):140-8
• Abstract PDF

• Ryan CM, Militello KT, Read LK.
• Polyadenylation regulates the stability of Trypanosoma brucei mitochondrial RNAs.
• J Biol Chem. 278(35):32753-62
• Abstract PDF

• Pelletier M, Read LK.
• RBP16 is a multifunctional gene regulatory protein involved in editing and stabilization of specific mitochondrial mRNAs in Trypanosoma brucei.
• RNA. 9(4):457-68
• Abstract PDF

2002

2001

• Hayman, M.L., M.M. Miller, D.M. Chandler, C.C. Goulah, and L.K. Read.
• The trypanosome homolog of human p32 interacts with RBP16 and stimulates its gRNA binding activity.
• Nucl. Acids Res. 29(24):5216-5225
• Abstract PDF

• Pelletier, M., Y. Xu, X. Wang, S. Zahariev, S. Pongor, J.M. Aletta, and L.K. Read.
• Arginine methylation of a mitochondrial guide RNA binding protein from Trypanosoma brucei.
• Mol. Biochem. Parasitol. 118(1):49-59
• Abstract PDF

2000

• Militello, K.T., M.L. Hayman, and L.K. Read.
• Transcriptional and post-transcriptional in organello labeling of Trypanosoma brucei mitochondrial RNA.
• Int. J. Parasitol. 30:643-647
• Abstract PDF

• Militello, K.T. and L.K. Read.
• UTP-dependent and UTP-independent pathways of mRNA turnover in Trypanosoma brucei mitochondria.
• Mol. Cell. Biol. 20(7):2308-2316
• Abstract PDF

• Pelletier, M., M.M. Miller, and L.K. Read.
• RNA binding properties of the mitochondrial Y-box protein RBP16.
• Nucl. Acids Res. 28(5):1266-1275
• Abstract PDF

1999

• Read, L.K., K.T. Militello, and G.E. Nerantzakis.
• Cloning and characterisation of a cDNA encoding the Trypanosoma brucei ribosomal protein L24.
• Int. J. Parasitol. 29(4):601-605
• Abstract PDF

• Hayman, M.L. and L.K. Read.
• Trypanosoma brucei RBP16 is a mitochondrial Y-box family protein with guide RNA binding activity.
• J. Biol. Chem. 274(17):12067-12074
• Abstract PDF

• Militello, K.T. and L.K. Read.
• Coordination of kRNA editing and polyadenylation in Trypanosoma brucei mitochondria: Complete editing is not required for long poly(A) tract addition.
• Nucl. Acids Res. 27(5):1377-1385
• Abstract PDF

• Hotchkiss, T.L., G.E. Nerantzakis, S.C. Dills, L. Shang, and L.K. Read.
• Trypanosoma brucei poly(A) binding protein I cloning, expression, and binding to 5' untranslated region sequence elements.
• Mol. Biochem. Parasitol. 98(1):117-129
• Abstract PDF