RecodeTryps is an online tool that can be used to recode Trypanosoma brucei protein coding sequence for optimal codon usage  or to generate RNA interference resistant sequence. The RNAi resistant sequence will not match the input sequence in more than nine nucleotides in a row while retaining the coding potential.
How to use RecodeTryps
Make sure the sequence you want to transform only contains in-frame full length codons. Any sequence not dividable by three will be rejected. Paste the nucleotide sequence into the text field. Any characters other than A T G or C will be automatically omitted. Choose whether you want to recode the sequence for optimal codon usage [Optimize] or to create an RNAi resistant fragment [Recode].
- ^ Horn D. 2008. Codon usage suggests that translational selection has a major impact on protein expression in trypanosomatids. BMC Genomics 9:2.