Transformation of Competent Bacteria

  1. Thaw bacteria on ice
  2. Add 2μl of DNA in a chilled 1.5ml eppie tube
  3. Place 30μl in tube with DNA
  4. Incubate on ice 30 min
  5. Heat shock for 45 sec at 42°C
  6. Place on ice for 2 min
  7. Add 500μl SOC and shake at 37°C for 1 hr
  8. Move 20μl of culture to a new tube and spin down the rest at 13,000 rpm for 1 min
  9. Resuspend both samples to 100μl SOC
  10. Plate on LB agar (+ appropriate antibiotic) overnight 37°C

Download Protocol: Transformation Protocol
(note requires Microsoft Word to open)