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Aspartate Aminotransferase (AST/GOT) Assay Kit

Components:

AST Assay Solution: 10 ml; store at -80°C (for 200 assays)

AST Substrate: 0.5 ml; store at -80°C

10X Cell Lysis Solution: 25 ml; store at 4°C

Product Description: Aspartate aminotransferase (AST) is a member of the transaminase family of enzymes. The enzyme is previously referred
to as glutamate oxaloacetate transaminase (GOT), which indicates its biological function in intermediary metabolism of glucose and protein
through catalyzing the transfer of an amino group between aspartate and glutamate. AST is found in many tissues throughout the body, including
the liver, heart, muscle, brain, and kidney, and therefore AST measurement along with ALT measurement is routinely performed in clinical
diagnostics. While serum levels of AST are elevated in most types of liver disease, increased serum AST may also be associated with myocardial
infarction, renal damage, and muscle necrosis. Assessment of the ratio of AST to ALT also provides clinically useful information. The colorimetric
AST assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and
exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased AST activity. The kit is
stable for at least one year if handled and stored properly. The product is for research use only.

Features: The non-radioactive assay kit allows the assay to be completed within 30 min with minimal pipetting steps. Each kit is sufficient for approximately 200 assays using the 96-well format. See related product ALT assay kit, GLDH assay kit, LDH assay kit, GAPDH assay kit, G6PD assay kit, PDH assay kit, Glucose assay kit, and Lactate Assay Kit. The method is adaptable to automation.