Our websites: www.acsu.buffalo.edu/~chunglee or www.bmrservice.com

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit (Cat #: E-101)

Components:     GAPDH Assay Solution: 5 ml, store at -20°C (100 microassays)

                                 10x Cell Lysis Solution: 25 ml, store at 4ºC

Product Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme mediating the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphate glycerate with the production of NADH. Although this house-keeping gene is often used as internal control for RNA and protein analysis, expression of the gene can be altered under certain cell growth and differentiation conditions. In addition, a switch from aerobic metabolism to anaerobic glycolysis may also be accompanied by a change in GAPDH expression. The GAPDH enzyme activity assay kit can be used to quickly assess potential changes in the expression of the gene. The non-radioactive colorimetric GAPDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased GAPDH activity. The kit is stable for at least one year if handled and stored properly. The product is for research use only.

  Features: The assay is simple, non-radioactive, and very sensitive. Each kit is sufficient for 100 microassays.