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Glucose Assay Kit

Components:

Glucose Assay Solution:         10 ml; store at -80°C
1 M Glucose Standard:          0.1 ml; store at 4°C

Product Description: Glucose, being a major energy source in most eukaryotic and prokaryotic organisms, is subject to tight metabolic regulation. In humans, glucose homeostasis under different metabolic circumstances is of vital importance, and insulin regulates the concentration of glucose in the blood such that its concentration normally falls within the 3.5-8 mM range. Abnormal circulating levels of glucose are associated with several pathologic states such as diabetes and obesity. Aerobic and anaerobic glucose metabolic pathways convert glucose to acetyl-CoA and lactic acid, respectively, which are differentially adopted by different tissues. Cell culture media typically contain high levels of glucose (5-25 mM), which promotes lactic acid formation and secretion in culture. The Glucose Assay Kit is based on sequential hexokinase and glucose-6-phosphate dehydrogenase reactions in a NADP+-coupled oxidation-reduction, which generates formazan exhibiting an absorption maximum at 492 nm. The Glucose Assay kit is used to measure the concentration of glucose present in diluted serum and cell culture media with a linear detection range of 0.1 – 1 mM. Sample pretreatment with TCA or PCA is not required. The assay solution is stable for at least one year, and should be kept at -80ºC for long term storage (more than 1 month). The product is for research use only.