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Alanine Aminotransferase (ALT/GPT) Assay Kit

Components:

ALT Assay Solution: 10 ml; store at -80°C (for 200 assays)

ALT Substrate: 0.25 ml; store at -80°C

10X Cell Lysis Solution: 25 ml; store at 4°C

Product Description: Alanine aminotransferase (ALT) is a member of the transaminase family of enzymes. The enzyme is previously referred to
as glutamate pyruvate transaminase (GPT), which indicates its biological function in intermediary metabolism of glucose and protein through
catalyzing the transfer of an amino group between alanine and glutamate. ALT is present at the highest level in the liver with smaller amounts of
the enzyme in the heart, muscle, and kidney, and as such its measurement constitutes part of the liver function tests. While marked elevation in
serum ALT may signal acute liver damage even prior to manifestation of the disease symptom, it can also be associated with cardiovascular and
muscle diseases.  Assessment of the ratio of AST to ALT also provides clinically useful information. The colorimetric ALT assay is based on the
reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum
at 492 nm. The intensity of the red color formed is increased in the presence of increased ALT activity. The kit is stable for at least one year if
handled and stored properly. The product is for research use only.

Features: The non-radioactive assay kit allows the assay to be completed within 30 min with minimal pipetting steps. Each kit is sufficient for approximately 200 assays using the 96-well format. See related product AST assay kit, GLDH assay kit, LDH assay kit, GAPDH assay kit, G6PD assay kit, PDH assay kit, Glucose assay kit, and Lactate Assay Kit. The method is adaptable to automation.