Park Group

Department of Chemical and Biological Engineering



26. Dundas et al. Directed evolution of monomeric streptavidin with improved kinetic properties (in preparation)

25. Mann, J.K., Park, S., Enhancement of NOD2 response to muramyl dipeptide stimulation by a peptide that targets the leucine rich repeats (submitted)

24. Demonte, D., Dundas, C.M., Park, S., Expression and purification of soluble monomeric streptavidin in E. coli. App Microbiol & Biotech 98, 6285-6295 (2014)
23. Mann, J.K., Park, S. Epitope specific binder design by yeast surface display (Meth Mol Biol, invited and submitted)

22. Dundas, C.M., Demonte, D., Park, S., Streptavidin-biotin technology: improvements and innovations in chemical and biological applications. App Microbiol & Biotech 97:9343-9353 (2013)
21. Park, S., Mann, J.K., Li, N., Targeted inhibitor design: lessons from small molecule drug design, directed evolution, and vaccine research. J SciMed Central CEPT 1:1004(2013). (read).
20. Demonte, D., Drake, E., Lim, K.H., Gulick, A., Park, S., Structure based engineering of streptavidin monomer with a reduced biotin dissociation rate. Proteins: Structure, Function, and Bioinformatics 81, 1621-1633 (2013)

19. Mann, J.K., Wood, J.F., Stephan, A.F., Tzanakakis, E.S., Ferkey, D.M., Park, S. Epitope guided engineering of monobody binders for in vivo inhibition of Erk-2 signaling. ACS Chem Biol 8, 608-616 (2013)

18. Lim, K.H., Huang, H., Pralle, A., and Park, S. Stable, High-Affinity Streptavidin Monomer for Protein Labeling and Monovalent Biotin Detection. Biotechnology Bioeng 110, 57-67 (2013)
(Video abstract available on youtube.)
(Plasmids available at Addgene: pRSET-mSA, pRSET-mSA-EGFP, pDisplay-mSA-EGFP-TM, pYD1-mSA.)

17. Lim, K.H., Huang, H., Pralle, A., and Park, S. Engineered streptavidin monomer and dimer with improved stability and function. Biochemistry 50, 8682-8691 (2011)
16. Lim, K.H., Hwang, I. and Park, S. Biotin-assisted folding of streptavidin on the yeast surface. Biotechnol Prog 28, 276-283 (2011)

15. Hsu, C.K. and Park, S. Computational and mutagenesis studies of the streptavidin native dimer interface. J Mol Graph Model 29, 295-308 (2010)
         -Featured as "Topical Perspectives"

14. Lim, K.H., Hsu, C.K., Park, S. Flow cytometric analysis of genetic FRET detectors containing variable substrate sequences. Biotechnol Progress 26, 1765-1771 (2010)
13. Lim, K.H., Madabhushi, S.R. (*), Mann, J. (*), Neelamegham, S., Park, S. Disulfide trapping of protein complexes on the yeast surface. Biotechnol Bioeng 106, 27-41 (2010). *Equal contributions.
         - Featured in Spotlight ("Getting STUCKED")

12. Sheldon Park and Jennifer Cochran (Eds). Protein Engineering and Design, CRC Press (2009)

11. Hwang, I. and Park, S. Computational design of protein therapeutics. Drug Disc Today: Tech 5, e43-48 (2008).

10. Szep,S.(*), Park, S.(*#), Boder, E. T., Van Duyne, G., Saven, J. G. Structural coupling between FKBP12 and buried water. Proteins: Structure, Function, and Bioinformatics 74, 603-11 (2009). (*) Equal contributions, (#) Corresponding author.

9. Park, S., Xu, Y., Stowell, X. F., Gai, F., Saven, J. G., Boder, E. T. Limitatioins of yeast surface display in engineering proteins of high thermostability. Protein Eng Des Sel (PEDS) 19, 211-217 (2006).

8. Park, S. & Saven, J. G. Simulation of pH-dependent edge strand rearrangement in human b-2 microglobulin. Protein Sci 15, 200-207 (2006).

7. Park, S. & Saven, J. G. Statistical and molecular dynamics studies of buried waters in globular proteins. Proteins 60, 450-463 (2005).

6. Park, S., Saven, J. G. Computationally assisted protein design. Annu Rep in Compu Chem, Volume 1, Chapter 18, pp245- 253 (2005).

5. Park, S., Boder, E. T., Saven, J. G. Modulating the DNA affinity of Elk-1 with computationally selected mutations. J Mol Biol 348, 75-83 (2005).

4. Park, S., Kono, H., Wang, W., Boder, E. T., Saven, J. G. Progress in the development and application of computational methods for probabilistic protein design. Comp Chem Eng 29, 407 (2005).

3. Park, S., Fu, X., Wang, W., Yang, X., Saven, J. G. Computational protein design and discovery. Annu Rep Prog Chem Sect C 100, 195-236 (2004).

2. Park, S., Yang, X., Saven, J. G. Advances in computational protein design. Curr Opin Struct Biol 14, 487-494 (2004).

1. Park, S., Uesugi, M., Verdine, G. L. A second calcineurin binding site on NFAT regulatory domain. Proc Natl Acad Sci U S A 97, 7130-5 (2000).


Sheldon Park and Kok Hong Lim, Compositions Comprising Monomeric Streptavidin and Methods for Using Same, US Patent filed (Application no. PCT/US10/32542)