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Hexokinase Assay Kit

Components:

Hexokinase Assay Solution: 10 ml; store at -20°C (for 200 assays)

10X Cell Lysis Solution: 25 ml; store at 4°C

Product Description: Hexokinase catalyzes the first step of glycolysis, converting glucose to glucose-6-phosphate. There are four mammalian hexokinase isoforms (I, II, III, and VI) that differ in tissue distribution and catalytic kinetics. Hexokinase I, II, and III exhibit a low Km for glucose whereas hexokinase IV (glucokinase) has a Km for glucose 100 times higher than that of other hexokinase. The hexokinase assay solution contains 20 mM glucose, and thus measures the total hexokinase activity of crude cell or tissue extracts. This non-radioactive colorimetric assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction. The INT reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased hexokinase activity. The enzyme assay solution should be kept at -80ºC for long-term storage (more than 1 month), and will remain stable indefinitely at -80ºC.  The product is for research use only.

Features: The non-radioactive assay kit allows the assay to be completed within 30 min with minimal pipetting steps. Each kit is sufficient for approximately 200 assays using the 96-well format. See related product LDH assay kit, GAPDH assay kit, G6PD assay kit, PDH assay kit, Glucose assay kit, and Lactate Assay Kit. The method is adaptable to automation.