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Aldehyde Dehydrogenase (ALDH) Assay Kit

Components:

ALDH Assay Solution: 10 ml; store at -80°C (for 200 assays)

10X Cell Lysis Solution: 25 ml; store at 4°C

Product Description: In mammals, ethanol is metabolized mainly in the liver by alcohol dehydrogenase (ADH), which oxidizes ethanol to acetaldehyde. Acetaldehyde, a toxic metabolite responsible for the miserable effects of hangovers, is further oxidized to acetate by aldehyde dehydrogenase (ALDH). ALDH belongs to a large family of aldehyde dehydrogenases that can be found in many tissues of the body, but are at the highest concentrations in the liver. ALDH1 and ALDH2 display different abilities to detoxify small aldehydes (such as acetaldehyde) and large aldehydes (such as retinaldehyde). The assay solution uses acetaldehyde as a substrate, and thus preferentially detects the activity of ALDH2 in crude extracts. This non-radioactive colorimetric assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction. The INT reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased ALDH activity. The enzyme assay solution should be kept at -80ºC, and will remain stable at -80ºC for ~1 year.  The product is for research use only.

Features: The non-radioactive assay kit allows the assay to be completed within 30 min with minimal pipetting steps. Each kit is sufficient for approximately 200 assays using the 96-well format. See related product LDH assay kit, GAPDH assay kit, G6PD assay kit, PDH assay kit, Glucose assay kit, and Lactate Assay Kit. The method is adaptable to automation.