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b-Galactosidase Staining Kit

Components:

            Xgal Buffer: 150 ml, store at 4ºC

            Ferricyanide: 10 ml, store at 4ºC

            Ferrocyanide: 10 ml, store at 4ºC

            Xgal: 4x1 ml, store at -20ºC

Product Description: The bacterial enzyme b-galactosidase (b-Gal) is a widely used reporter enzyme, the activity of which can be conveniently measured spectrophotometrically (J. Med. Chem. 7:574,1964). The b-Gal Staining Kit is based on the histochemical substrate 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (Xgal) for in situ staining of transgenic tissues or monolayer cells transiently or stably expressing b-Gal (see b-Galactosidase Assay Kit). The cells expressing b-Gal stain blue after mild fixation and substrate exposure. The product is for research use only.

Features: The staining kit requires minimal steps for staining b-Gal. This method provides rapid information regarding exogenous expression of b-Gal in animal tissue and transfection efficiency in cell culture. Note that a 4% paraformaldehyde solution is required for cell fixation, but is not included in the kit.