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b-Galactosidase Assay Kit

Components: 

ONPG Solution: 40 ml (400 assays), store in aliquots at -20ºC

10x Cell Lysis Solution: 25 ml, store at 4ºC

Stop Solution: 25 ml, store at 4ºC

Product Description: The bacterial enzyme b-galactosidase (b-Gal) is a widely used reporter enzyme, the activity of which can be conveniently measured spectrophotometrically (J. Med. Chem. 7:574,1964). The b-Gal assay kit utilizes the spectroscopically active compound o-nitrophenyl-b-D-galactopyranoside (ONPG) for quantitative measurements of b-Gal activity in cells (higher eukaryotes, yeast, or bacteria) transfected or transformed with b-Gal reporter DNA (see b-Galactosidase Staining Kit). The enzyme converts ONPG to o-nitrophenol, which exhibits an extinction coefficient of 4,500 M^-1cm^-1 at 420 nm. The stability of our specially formulated substrate solution is guaranteed for as least 1 year from the time of purchase if handled and stored properly. The product is for research use only.

Features: Each assay kit includes 40 ml of optimized ONPG Substrate Solution sufficient for 400 assays in microplate formats. Cell Lysis Solution is compatible with luciferase (see Luciferase Assay Kit) and CAT enzyme assays permitting flexibility and maximum sensitivity. Substrate is stable for at least one year if handled and stored properly. The method is adaptable to automation.