Gulick Laboratory

2023

Patel, K.D. and Gulick, A.M. (2023) Structural and functional insights into δ-poly-L-ornithine polymer biosynthesis from Acinetobacter baumannii. Communications Biology 6, 982.

Patel, K.D., Ahmed, S.F., MacDonald, M. R., and Gulick, A.M. (2023) Structural studies of modular nonribosomal peptide synthetases. Methods in Molecular Biology 2670, 17-48.

Patel, K.D., MacDonald, M.R., Ahmed, S.F., Singh, J., and Gulick, A.M. (2023) Structural advances toward understanding the catalytic activity and conformational dynamics of modular nonribosomal peptide synthetases. Natural Products Reports 40, 1550-1582.

2022

Yang, J., Banas, V.S., Patel, K.D., Rivera, G., Mydy, L.S., Gulick, A.M., and Wencewicz, T.A. (2022) An acyl-adenylate mimic reveals the structural basis for substrate recognition by the iterative siderophore synthetase DesD. J. Biol. Chem. 298(8) 102166.

2021

Patel, K.D. and Gulick, A.M. (2021) Anti-Zika candidates from a marine fungus with a remarkable biosynthetic repertoire. J. Biol. Chem. 297, 101047.

Swayambhu, G., Bruno, M., Gulick, A.M., and Pfeifer, B. A. (2021) Siderophore natural products as pharmaceutical agents. Curr Opin in Biotechnol 69, 242-251.

Koeller, C.M., Smith, T.K., Gulick, A.M., and Bangs, J.D. (2021) p67: A cryptic lysosomal hydrolase in Trypanosoma brucei? Parasitology 148, 1271-1276.

2020

Mhashal, A.R., Romero-Rivera, A., Mydy, L.S., Cristobal, J.R., Gulick, A.M., Richard, J.P., and Kamerlin, S.C.L. (2020) Modeling the role of a flexible loop and active site side chains in hydride transfer catalyzed by glycerol-3-phosphate dehydrogenase. ACS Catalysis 10, 11253-11267.

Shi, C., Miller, B.R., Alexander, E.M., Gulick, A.M., and Aldrich, C.C. (2020) Design, synthesis, and biophysical evaluation of mechanism-based probes for condensation domains of nonribosomal peptide synthetases. ACS Chemical Biology 15, 1813-1819.

Alexander, E.M., Kreitler, D.F., Guidolin, V., Hurben, A.K., Drake, E.J., Villalta, P.W., Balbo, S., Gulick, A.M., and Aldrich, C.C. (2020) Biosynthesis, mechanism of action, and inhibition of the enterotoxin tilimycin produced by the opportunistic pathogen Klebsiella oxytoca. ACS Infectious Diseases 6, 1976-1997.

Mydy, L.S., Bailey, D.C., Patel, K.D., Rice, M.R., and Gulick, A. M. (2020) The Siderophore Synthetase IucA of the Aerobactin Biosynthetic Pathway Uses an Ordered Mechanism. Biochemistry 59, 2143-2153.

2019

Branchini, B. R., Fontaine, D. M., Southworth, T. L., Huta, B. P, Racela, A., Patel, K. D., and Gulick, A. M. (2019) Mutagenesis and structural studies reveal the basis for the activity and stability properties that distinguish the Photinus luciferases scintillans and pyralis. Biochemistry 58, 4293-4203.

Patel, K. D., d'Andrea, F. B., Gaudelli, N. M., Buller, A. R., Townsend, C. A., and Gulick, A. M. (2019) Structure of a bound peptide phosphonate reveals the mechanism of nocardicin bifunctional thioesterase epimerase-hydrolase half-reactions. Nature Communications 10, 3868.

Kreitler, D. F., Gemmell, E. M., Schaffer, J. E., Wencewicz, T. A., and Gulick, A. M. (2019) The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase. Nature Communications 10, 3432.

Russo, T. A. and Gulick, A. M. (2019) Aerobactin synthesis proteins as antivirulence targets in hypervirulent Klebsiella pneumoniae. ACS Infectious Diseases 5, 1052-1054.

Gulick, A. M. (2019) Engineered enzymes set a trap. Nature 565, 28-29.

Mydy, L. S., Cristobal, J. R., Katigbak, R. D., Bauer, P., Reyes, A. C., Kamerlin, S. C. L., Richard, J. P., and Gulick, A. M. (2019) Human glycerol 3-phosphate dehydrogenase: X-ray crystal structures that guide the interpretation of mutagenesis studies. Biochemistry 58, 1061-1073.

2018

Bailey, D. C., Alexander, E., Rice, M. R., Drake, E. J., Mydy, L. S., Aldrich, C. C., and Gulick, A. M. (2018) Structural and functional delineation of aerobactin biosynthesis in hypervirulent Klebsiella pneumoniae. J. Biol. Chem. 293, 7841-7852.

Ray, S., Kreitler, D. F., Gulick, A. M., and Murkin, A. S. (2018) The nitro group as a masked electrophile in covalent enzyme inhibition. ACS Chem Biol 13, 1470-1473.

Bailey, D.C., Buckley, B. P., Chernov, M. V, and Gulick, A. M. (2018) Development of a High-Throughput Biochemical Assay to screen for inhibitors of aerobactin synthetase IucA. SLAS Discovery 23, 1070-1082.

Gulick, A. M. and Aldrich, C. C. (2018) Trapping interactions between catalytic domains and carrier proteins of modular biosynthetic enzymes with chemical probes. Natural Product Reports, 35, 1156-1184.

Bailey, D. C., Bohac, T. J., Shapiro, J. A., Giblin, D. E., Wencewicz, T. A., and Gulick, A. M. (2018) Crystal structure of the siderophore binding protein BauB bound to an unusual 2:1 complex between acinetobactin and ferric iron. Biochemistry, 57, 6653-6661.

2017

Clevinger, K. D., Mascarenhas, R., Catlin, D., Wu, R., Kelleher, N. L. Drake, E. J. Gulick, A. M., Liu, D., and Fast W. (2017) Substrate trapping in the siderophore tailoring enzyme PvdQ. ACS Chem Biol 12, 643-647.

Branchini, B. R., Southworth, T. L., Fontaine, D. M., Murtiashaw, M. H., McGurk, A., Talukder, M. H., Qureshi, R., Yeetil, D., Sundlov, J. A., and Gulick, A. M. (2017) Cloning of the orange light-producing luciferase from Photinus scintillans provides insight into bioluminescence color determination. Photochemistry and Photobiology 93, 479-485.

Gulick, A. M. (2017) Nonribosomal peptide synthetase biosynthetic clusters of ESKAPE pathogens. Natural Products Reports 34, 981-1009.

2016

Drake, E. J., Miller, B. M., Shi, C., Tarrasch, J. T., Sundlov, J. A., Allen, C. L., Skiniotis, G., Aldrich, C. C., and Gulick, A. M. (2016) Structures of two distinct conformations of holo-non-ribosomal peptide synthetases. Nature 539, 235-238.

Miller, B. M. and Gulick, A. M. (2016) Structural biology of nonribosomal peptide synthetases. Methods in Molecular Biology 1401, 3-29.

Drake, E. J. and Gulick, A. M. (2016) 1.2A Crystal structure of periplasmic aminotransferase PvdN from Pseudomonas aeruginosa Acta Cryst F 72, 403-408.

Richard, J. P., Amyes, T. L., Malabanan, M. M., Zhai, X., Kim, K. J., Reinhardt, C. J., Wierenga, R. K., Drake, E. J., and Gulick, A. M. (2016) Structure-function studies on hydrophobic residues that clamp a basic glutamate side-chain during catalysis by triosephosphate isomerase. Biochemistry 55, 3036-3047.

Bailey, D.C., Drake, E. J., Grant, T. D., and Gulick, A. M. (2016) Structural and functional characterization of aerobactin synthetase IucA from a hypervirulent pathotype of Klebsiella pneumoniae. Biochemistry 55, 3559-3570.

Meneely, K. M., Sundlov, J. A., Gulick, A. M., Moran, G. R., and Lamb, A. L. (2016) An open and shut case: The interaction of Magnesium with MST Enzymes. JACS 138, 9277-9293.

Miller, B. R., Drake, E. J., Shi, C., Aldrich, C. C., and Gulick, A. M. (2016) Structures of a nonribosomal peptide synthetase module bound to MbtH-like proteins support a highly dynamic domain architecture. JBC 291, 22559-22571.

Gulick, A. M. (2016) Structural insight into the necessary conformational changes of modular nonribosomal peptide synthetases. Curr. Opinion Chemical Biology 35, 89-96.

2015

Branchini, B. R., Behney, C. E., Southworth, T. L. Fontaine, D. M., Gulick, A. M., Vinyard, D. J., and Brudvig, G. W. (2015) Experimental support for a single electron transfer oxidation mechanism in firefly luciferase. JACS 137, 7592-7595.

Zhu, J., Lippa, G. M., Gulick, A. M., and Tipton, P. A. (2015) Examining reaction specificity in PvcB, a source of diversity in isonitrile containing natural products. Biochemistry 54, 2659-2669.

Kholodar, S. A., Allen, C. L., Gulick, A. M., and Murkin, A. S. (2015) The role of phosphate in a multistep enzymatic reaction: reactions of the substrate and intermediate in pieces. JACS 137, 2748-2756.

Mitchell, C. A., Tucker, A. C., Escalante-Semerena, J. C., and Gulick, A. M. (2015) The Structure of S. lividans Acetoacetyl-CoA synthetase shows a novel interaction between the C-terminal extension and the N-terminal domain. Proteins 83, 575-581.

2014

Miller, B. R., Sundlov, J. A., Drake, E. J., Makin, T. A., and Gulick, A. M. (2014) Analysis of the linker region joining the adenylation and carrier protein domains of the modular non-ribosomal peptide synthetases. Proteins 82, 2691-2702.

Kholodar, S. A., Tombline, G., Liu, J., Tan, Z., Allen, C. L., Gulick, A. M., and Murkin, A. S. (2014) Alteration of the flexible loop in 1-deoxy-D-xylulose-5-phosphate reductoisomerase boosts enthalpy-driven inhibition by fosmidomycin. Biochemistry 53, 3423-3431.

Wurst, J. M., Drake, E. J., Theriault, J. R., Jewett, I. T., VerPlank, L., Perez, J. R., Dandapani, S., Palmer, M., Moskowitz, S. M., Schreiber, S. L., Munoz, B., and Gulick, A. M. (2014) Identification of Inhibitors of PvdQ, an enzyme involved in the synthesis of the siderophore pyoverdine. ACS Chemical Biology 9, 1536-1544.

Allen, C. L. and Gulick, A. M. (2014) Structural and bioinformatic characterization of an Acinetobacter baumannii type II carrier protein. Acta Cryst D, 70, 1718-1725

Russo, T. A., Olson, R., MacDonald, U., Metzger, D., Maltese, L., Drake, E. J., and Gulick, A. M. (2014) Aerobactin mediates virulence and accounts for the increased siderophore production under iron limiting conditions by hypervirulent (hypermucoviscous) Klebsiella pneumoniae. Infection and Immunity 82, 2356-2367.

2013

Theriault, J. R., Wurst, J., Jewett, I., Verplank, L., Perez, J. R., Gulick, A. M., Drake, E. J., Palmer, M., Moskowitz, S., Dasgupta, N., Brannon, M. K., Dandapani, S., Munoz, B. and Schreiber, S. (2013) Identification of a small molecule inhibitor of Pseudomonas aeruginosa PvdQ acylase, an enzyme involved in siderophore pyoverdine synthesis. Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD):National Center for Biotechnology Information (US).

DeMonte, D., Drake, E. J., Lim, K. H., Gulick, A. M., and Park, S. (2013) Structure based engineering of streptavidin monomer with reduced biotin dissociation rate. Proteins 81, 1621-1633.

Sundlov, J. A. and Gulick, A. M. (2013) Structure determination of the functional domain interaction of a chimeric Non-Ribosomal Peptide Synthetase from a challenging crystal with non-crystallographic translational symmetry. Acta Cryst D 69, 1482-1492.

Neres, J. P., Engelhart, C. A., Drake, E. J., Wilson, D. J., Fu, P., Boshoff, H. I., Barry III, C. E., Gulick, A. M., and Aldrich, C. C. (2013) Non-nucleoside inhibitors of BasE, an adenylating enzyme in the siderophore biosynthetic pathway of the opportunistic pathogen Acinetobacter baumannii. J. Med. Chem. 56, 2385-2405.

Tessier, M. B., Grant, O. C., Heimburg-Molinaro, J., Smith, D., Jadey, S., Gulick, A. M., Glushka, J., Deutscher, S. L., Rittenhouse-Olson, K., and Woods, R. J. (2013) Computational screening of the human TF-glycome provides a structural definition for the specificity of anti-tumor antibody JAA-F11. PLoS ONE 8(1), e54874.

Wilson, D. J., Shi, C., Teitelbaum, A. M., Gulick, A. M., and Aldrich, C. C. (2013) Characterization of AusA: A dimodular nonribosomal peptide synthetase responsible for production of aureusimine pyrazinones. Biochemistry 52, 926-937.

Reviews

Patel, K.D., Fisk, M.B., and Gulick, A.M. (2025) Discovery, functional characterization, and structural studies of the NRPS-independent siderophore synthetases. Critical Reviews in Biochemistry and Molecular Biology, in press.

Patel, K.D., Ahmed, S.F., MacDonald, M. R., and Gulick, A.M. (2023) Structural studies of modular nonribosomal peptide synthetases. Methods in Molecular Biology 2670, 17-48.

Patel, K.D., MacDonald, M.R., Ahmed, S.F., Singh, J., and Gulick, A.M. (2023) Structural advances toward understanding the catalytic activity and conformational dynamics of modular nonribosomal peptide synthetases. Natural Products Reports, 40, 1550-1582.

Swayambhu, G., Bruno, M., Gulick, A.M., and Pfeifer, B. A. (2021) Siderophore natural products as pharmaceutical agents. Curr Opin in Biotechnol 69, 242-251.

Koeller, C.M., Smith, T.K., Gulick, A.M., and Bangs, J.D. (2021) p67: A cryptic lysosomal hydrolase in Trypanosoma brucei? Parasitology 148, 1271-1276.