Regions upstream of the ribosome binding site strongly affect chloroplast translation in vivo
Chloroplast transformation is used to investigate the function of 5'UTRs in vivo
Target Plants (Week 2)
Antibiotic-resistant transformants (Week 6)
Second Selection (Week 10)
Rooting (Week 12)
Roots
Diagrams of the two 5'UTRs used in these experiments
5'UTR:
Wild-type
holo-sub
Con1
Con 2
RBS
RBS
Measuring the effects of two different 5'UTRs on RNA abundance and translation
Con 1 and Con 2 refer to phylogenetically conserved regions in the atpI 5'UTR. RBS is the abbreviation for ribosome binding site. Hatched boxes are non-chloroplast sequence. White boxes are wild-type chloroplast sequence.