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Pyruvate Assay Kit

Components:

Pyruvate Assay Solution: 10 ml; store at -20°C or -80°C (for 200 microassays)

40 mM Pyruvate Standard: 0.5 ml; store at -20°C

Product Description: Pyruvate is metabolized aerobically to acetyl-CoA and anaerobically to lactate (homolactic fermentation) or ethanol (alcoholic fermentation in yeast). Pyruvate is also converted to oxaloacetate by an anaplerotic reaction mediated by pyruvate carboxylase, and the oxaloacetate can be used for replenishing TCA cycle intermediates or promoting gluconeogenesis. Conversion of pyruvate to lactate is obligatory for bacteria, serving to regenerate NAD+, and is enhanced in eukaryotic cells when mitochondrial function is impaired. LDH-A-mediated conversion of pyruvate to lactate by cancer cells in the presence of adequate oxygen supply (aerobic glycolysis) constitutes the central concept of the Warburg hypothesis, and is believed to be the underlying cause of tumorigenesis. The Pyruvate Assay Kit is based on the difference in the UV absorption spectra between the oxidized and reduced forms of NAD+/NADH at 340 nm. As low as ~10 uM pyruvate may be detected by the assay kit in 30 min. The assay solution should be stored in aliquots at -80ºC, and will remain stable indefinitely at -80ºC. The product is for research use only.

See related product LDH assay kit, GAPDH assay kit, G6PD assay kit, PDH assay kit, NAD+ assay kit, and Glucose assay kit.