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NAD+ Assay Kit

Components:

NAD+ Assay Solution: 10 ml; store at -20°C or -80°C (for 200 microassays)

80 mM NAD+ Standard: 0.5 ml; store at -20°C

Product Description: In metabolism, the compound nicotinamide adenine dinucleotide accepts or donates electrons in redox reactions, forming the NAD+/NADH pair with a redox potential of -0.32 volts. The NAD+/NADH ratio is an important measurement that reflects both the metabolic and health states of cells. Key enzymes regulating the NAD+/NADH ratio are those dehydrogenases involved in fuel metabolism (such as lactate dehydrogenase and pyruvate dehydrogenase) and electron transport chain complexes (such as NADH dehydrogenase). Non-redox roles of NAD+ have received increasing attention in recent years. For instance, NAD+ is consumed in ADP-ribosylation reactions mediated by
ADP-ribosyltransferase and poly(ADP-ribose) polymerase. Sirtuins, a group of NAD-dependent deacetylases, also consume NAD+, and this enzymatic activity appears to be critically involved in the aging of vertebrates and invertebrates. The NAD+ Assay Kit is based on the difference in the UV absorption spectra between the oxidized and reduced forms of NAD+/NADH at 340 nm. As low as ~20 uM NAD+ extracted from cells or tissues may be detected by the assay kit in 30 min. The smallest assay volume (15 ul sample mixed with 50 ul NAD+ Assay Solution) requires a 50-ul Quartz cell for measuring changes in O.D.340 nm. However, the reaction can be proportionally scaled up using a larger Quartz
cuvette.The assay solution should be stored in aliquots at -80ºC, and will remain stable indefinitely at -80ºC. The product is for research use only.

See related product LDH assay kit, GAPDH assay kit, G6PD assay kit, PDH assay kit, Pyruvate assay kit, and Glucose assay kit.