Our websites: www.acsu.buffalo.edu/~chunglee or www.bmrservice.com

Lactate Dehydrogenase (LDH) Assay Kit

Components:

LDH Assay Solution: 10 ml; store at -20°C (for 200 microassays)

10X Cell Lysis Solution: 25 ml; store at 4°C

Product Description: Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. It consists of 4 subunits which may be of 2 different types: M (muscle) and H (heart), formerly known as A and B respectively. Five different isoenzymes are therefore possible, depending on the subunit composition. LDH is most often measured to evaluate the presence of tissue or cell damage. The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased LDH activity. The kit is stable for at least one year under proper storage and handling conditions. The product is for research use only.

Features: The superior sensitivity of the non-radioactive assay kit allows the assay to be completed within 20-30 min with minimal pipetting steps. Each kit is sufficient for approximately 200 assays using the 96-well format. See related product LDH assay kit, GAPDH assay kit, G6PD assay kit, PDH assay kit, Glucose assay kit, and Lactate Assay Kit. The method is adaptable to automation.