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ATP Assay Kit

Components:

ATP Assay Solution: 2x10 ml, store at -80ºC

1 mM ATP Standard: 0.5 ml, store at -20ºC or -80ºC

Product Description: Intracellular ATP concentrations vary during cell growth and cell death. Notably, cells maintain a critical threshold of ATP levels, which dictates whether apoptotic or necrotic cell death may take place in conditions of stress. Evaluation of cellular ATP levels may thus provide useful information regarding the metabolic state and integrity of the cell. The enzyme luciferase catalyzes, in the presence of ATP (see Luciferase Assay Kit), the oxidation of luciferin with concomitant emission of yellow-green light, which can be conveniently measured by scintillation counters or luminometers. Light emission peaks in several seconds at 560 nm when the reaction is conducted at pH 7.8 (Anal. Biochem. 80:496,1977). The rapid appearance and decay of the light flash requires consistent timing of the light measurement to obtain reliable data. This provides the basis for a sensitive non-radioactive ATP assay system more convenient than that based on HPLC. The kit is sufficient for 200 assays using 0.1 ml of ATP Assay Solution per assay. The ATP Assay Solution if stored in aliquots at -75°C is stable for many years. Repeated freeze-thaw cycles should be avoided. The product is for research use only.