pCHNFI-A1.1 up to 3 cutters Sequence Range: 1 to 5305 >SphI | >EcoRI >BspMI | >Sse8387I | | | | | | >start_of_CMV_promoter/enhancer | | | | | | | | | | 60 GAATTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC 120 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG 180 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG >NdeI | | 240 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA 300 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC >BsaA1 >NcoI | | >SnaBI >StyI | | | | 360 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 420 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 480 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA >SacI | >TATA_box_start_of_CMV_promoter | | | | 540 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA >BpmI >BbsI | | >presumed_start_site_of_CMV_promoter | | | | | | | 600 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC >BspE1 >XbaI >XhoI | | | >end_of_CMV_promoter_XbaI_site >start_of_SV40_splice_region | | | | | | | | | | 660 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TCGAGGAACT GAAAAACCAG >HpaI >EcoO109I | | >start_of_16S_RNA_intron >SV40_deletion_site_BamHI_site | | | | | | | | 720 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG >StuI | >last_base_of_16S_RNA_intron || || 780 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA >SacII | >NotI | >EagI | >end_of_SV40_sequence >NotI_cloning_site | | | | 840 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GCCGCCAGCC ATGTATCCTT M Y P> >BciVI >SfiI | | | | 900 ACGATGTACC AGACTATGCT GGGCCTCACG GGCCGATGTA TTCTCCGCTC TGTCTCACCC Y D V P D Y A G P> H G P M Y S P L C L T> >XmnI | | 960 AGGATGAGTT TCATCCTTTC ATTGAAGCAC TTCTGCCCCA TGTCCGCGCC TTCGCCTACA Q D E F H P F I E A L L P H V R A F A Y> >BspMI | >Sse8387I >XmnI | | | | | | 1020 CATGGTTCAA CCTGCAGGCC CGAAAGCGGA AATACTTCAA AAAACATGAG AAGCGCATGT T W F N L Q A R K R K Y F K K H E K R M> >BpuI102I | | 1080 CGAAAGAAGA GGAGAGGGCC GTGAAGGATG AACTGCTAAG CGAGAAGCCC GAGGTCAAGC S K E E E R A V K D E L L S E K P E V K> >MscI >EcoRV | | | | 1140 AGAAGTGGGC TTCCCGACTT CTGGCCAAGT TACGGAAAGA TATCCGACCC GAGTACCGAG Q K W A S R L L A K L R K D I R P E Y R> >BclI | 1200 AGGATTTTGT TCTTACAGTT ACAGGGAAAA AACCTCCATG CTGTGTTCTT TCCAACCCTG E D F V L T V T G K K P P C C V L S N P> >EcoNI | | 1260 ATCAGAAAGG CAAGATGCGG AGAATTGACT GCCTCCGCCA GGCAGATAAA GTATGGAGGT D Q K G K M R R I D C L R Q A D K V W R> >Tth111I >ScaI | | | | 1320 TGGACCTCGT CATGGTGATC TTGTTCAAAG GTATTCCGCT GGAAAGTACT GATGGCGAGC L D L V M V I L F K G I P L E S T D G E> 1380 GCCTTGTAAA GAGTCCACAG TGCTCTAATC CAGGGCTCTG TGTCCAGCCC CATCACATAG R L V K S P Q C S N P G L C V Q P H H I> >BsrGI | | 1440 GGGTTTCTGT AAAGGAACTC GATTTATATT TGGCATACTT TGTACATGCA GCAGATTCAA G V S V K E L D L Y L A Y F V H A A D S> >PflMI | >XcmI| | | | 1500 GTCAATCTGA AAGTCCCAGC CAGCCAAGTG AAGCTGACAT TAAGGACCAG CCAGAAAATG S Q S E S P S Q P S E A D I K D Q P E N> >DrdI | | 1560 GACATTTGGG CTTCCAGGAC AGCTTTGTCA CATCAGGTGT TTTCAGTGTG ACTGAGCTAG G H L G F Q D S F V T S G V F S V T E L> 1620 TAAGAGTGTC ACAAACACCA ATAGCTGCAG GAACCGGCCC CAATTTTTCT CTCTCTGATT V R V S Q T P I A A G T G P N F S L S D> >BplI >BsrDI >BsaI | | | | | | 1680 TGGAAAGTTC TTCATACTAC AGCATGAGTC CAGGAGCAAT GAGGAGGTCT CTGCCCAGCA L E S S S Y Y S M S P G A M R R S L P S> >BplI | | 1740 CATCCTCTAC CAGCTCTACA AAGCGCCTCA AGTCTGTGGA GGATGAAATG GACAGTCCTG T S S T S S T K R L K S V E D E M D S P> >StyI >PflMI >BstXI | | | | | 1800 GTGAAGAACC ATTTTACACA GGCCAAGGGC GCTCCCCAGG GAGTGGCAGC CAGTCCAGTG G E E P F Y T G Q G R S P G S G S Q S S> >PflMI | | 1860 GATGGCATGA AGTAGAGCCA GGCTTGCCAT CTCCAAGCAC ACTGAAGAAG TCTGAGAAGT G W H E V E P G L P S P S T L K K S E K> >Eco57I >BsmI | | | | 1920 CTGGTTTCAG CAGCCCCTCC CCTTCGCAGA CCTCCTCCCT GGGAACAGCA TTCACACAGC S G F S S P S P S Q T S S L G T A F T Q> >EcoO109I | | 1980 ATCACCGACC TGTCATTACA GGACCCAGAG CAAGTCCACA TGCGACGCCA TCGACTCTCC H H R P V I T G P R A S P H A T P S T L> >BstXI | | 2040 ACTTTCCAAC GTCACCCATC ATCCAGCAGC CTGGGCCTTA CTTCTCACAC CCAGCCATCC H F P T S P I I Q Q P G P Y F S H P A I> >Bsu36I | | 2100 GTTACCACCC TCAGGAGACG CTGAAAGAGT TTGTCCAACT TGTCTGTCCT GATGCTGGTC R Y H P Q E T L K E F V Q L V C P D A G> 2160 AGCAAGCTGG ACAGGTGGGG TTCCTCAATC CCAATGGAAG CAGTCAAGGC AAGGTGCACA Q Q A G Q V G F L N P N G S S Q G K V H> >StuI | >MscI | | | | |2220 ACCCATTCCT CCCCACCCCA ATGTTGCCGC CGCCGCCACC ACCACCGATG GCCAGGCCTG N P F L P T P M L P P P P P P P M A R P> 2280 TGCCTCTGCC CATGCCAGAC ACCAAGCCTC CAACCACATC AACAGAAGGA GGTGCAGCCT V P L P M P D T K P P T T S T E G G A A> >BsgI | | 2340 CCCCCACCTC ACCGACCTAC TCGACACCCA GCACCTCCCC CGCAAACCGA TTCGTCAGTG S P T S P T Y S T P S T S P A N R F V S> >HindIII >Tth111I >KpnI | | | | | |2400 TTGGACCACG GGATCCAAGC TTTGTAAATA TCCCTCAACA GACACAGTCC TGGTACCTGG V G P R D P S F V N I P Q Q T Q S W Y L> >NheI >SacII | | | | >BamHI | | | | >start_of_SV40_polyA_region | | | | | | | | 2460 GATAAAAGTT GCAGCATCCC GCATAGCTAG CTAGGGCCGC GGGGATCCAG ACATGATAAG G *> >BsmI | | 2520 ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG >HpaI | 2580 TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA >MunI >BsmI | | | | 2640 CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTC >SphI | >AccI >BspMI | >HindIII | | | | >XbaI >SalI >Sse8387I | | | || | | | | >end_of_SV40_polyA_region | | | | | | || | | | | | | || | | | | 2700 GGATCCTCTA GAGTCGACCT GCAGGCATGC AAGCTTGGCG TAATCATGGT CATAGCTGTT 2760 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA >VspI | | 2820 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT >PvuII >VspI | | | | 2880 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC >SapI | | 2940 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG 3000 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC >AflIII | >AflIII | >BspLU11I | | 3060 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 3120 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA >DrdI | | 3180 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA >BssSI >BciVI | | | | 3240 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 3300 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 3360 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 3420 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA >AlwNI | | 3480 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 3540 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT >Eco57I | | 3600 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 3660 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 3720 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG >RcaI | | 3780 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA >DraI >DraI | | | | 3840 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG 3900 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG >EamI105I | | 3960 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC >BsaI | >BsrDI >BpmI | | | | 4020 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC 4080 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC >VspI | | 4140 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG >Psp1406I | >FspI | >BsrDI | | | | | | 4200 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT 4260 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG >PvuI | | 4320 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT 4380 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG >ScaI >BcgI | | | | 4440 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG >Bcg1 >DraI | | | 4500 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT >Psp1406I | >XmnI | | 4560 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT >Eco57I | >BssSI | | | | 4620 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC 4680 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT >SspI | | 4740 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT >BciVI | >RcaI | | | | 4800 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA 4860 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT >EcoO109I | >RcaI >BssSI | | | | | | | 4920 TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT 4980 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT >DrdI | | 5040 GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG >BstAPI | >NdeI || || 5100 TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG >NarI | | 5160 GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT >FspI >PvuI >PvuII | | | | | 5220 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT 5280 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGT Sequence Range: 1 to 5305 Enzyme #Cuts Positions AccI 1 2654 AflIII 2 3030 3030 AlwNI 1 3446 BbsI 1 600 Bcg1 1 4462 BcgI 1 4428 BciVI 3 844 3233 4760 BclI 1 1199 BplI 2 1652 1684 BpmI 2 579 3993 BpuI102I 1 1056 BsaA1 1 306 BsaI 2 1672 3984 BsgI 1 2293 BsmI 3 1908 2499 2592 BspE1 1 617 BspLU11I 1 3030 BspMI 3 12 979 2666 BsrDI 3 1663 3984 4158 BsrGI 1 1421 BssSI 3 3203 4587 4894 BstAPI 1 5095 BstXI 2 1797 2010 Bsu36I 1 2050 DraI 3 3789 3808 4500 DrdI 3 1524 3138 5007 EagI 1 819 EamI105I 1 3923 Eco57I 3 1863 3578 4590 EcoNI 1 1236 EcoO109I 3 702 1941 4899 EcoRI 1 1 EcoRV 1 1121 FspI 2 4145 5168 HindIII 2 2357 2671 HpaI 2 666 2578 KpnI 1 2396 MscI 2 1104 2211 MunI 1 2587 NarI 1 5146 NcoI 1 326 NdeI 2 200 5094 NheI 1 2426 NotI 1 819 PflMI 3 1497 1782 1801 Psp1406I 2 4149 4522 PvuI 2 4293 5189 PvuII 2 2854 5218 RcaI 3 3750 4758 4863 SacI 1 534 SacII 2 819 2440 SalI 1 2653 SapI 1 2914 ScaI 2 1307 4403 SfiI 1 869 SnaBI 1 306 SphI 2 17 2669 Sse8387I 3 23 976 2663 SspI 1 4727 StuI 2 769 2216 StyI 2 326 1763 Tth111I 2 1266 2384 VspI 3 2801 2860 4095 XbaI 2 624 2647 XcmI 1 1494 XhoI 1 640 XmnI 3 928 993 4522