Our laboratory is currently investigating the mechanisms by which various inflammatory and immune molecules and their respective receptors activate and regulate the immune response to various pathogenic microorganisms. In particular, we are investigating various genetic polymorphisms in the genes which encode for these molecules, and how these polymorphism may affect their normal functions and/or are associated with susceptibility to disease. Molecules under study include various inflammatory cytokines and their receptors as well as fibrinogen and other acute phase proteins. Our laboratory is also studying the function of neutrophils, which are primary phagocytic cells that play a key role in host defense against extracellular bacteria, as well as participate in the acute phase of inflammatory reactions. Funding for research is provided by grants from the National Institute of Health.
Boehm, T.K., E. De Nardin. Fibrinogen binds IgG antibody and enhances IgG-mediated phagocytosis. 2008. Hum. Antibodies (in press).
Sahingur, E, T.K. Boehm, H.T. Sojar, A. Sharma, and E. De Nardin. 2006 Fibrinogen-Neutrophil Interactions in response to fMLP and Porphyromonas gingivalis Fimbrial peptides. Immunol. Invest. 35:63-74.
Hajishengallis G, Sojar H, Genco RJ, and De Nardin E. 2004. Intracellular signaling and cytokine induction upon interactions of Porphyromonas gingivalis fimbria with pattern-recognition receptors. Immunol. Invest. 33, 1–16.
Yamamoto K, Kobayashi T, Ho A, Genco RJ, Yoshie H, and De Nardin E. 2004. Association of Fcg Receptor IIa Genotype with Chronic Periodontitis in Caucasians. J. Periodontol., 75:517-522.
Sahingur, SE, Genco, RJ, Sharma, A, and De Nardin, E. 2003. Association of Increased Levels of Fibrinogen and the –455G/A Fibrinogen Gene Polymorphism with Chronic Periodontitis. J. Periodontol. 74:329-337.
Hajishengallis G, Martin M, Hakimuddin S, Sharma A, Schifferle RE, Denardin E, Genco RJ. 2002. Dependence of bacterial protein adhesions on toll-like receptors for proinflammatory cytokine induction. Clin. Diagnostic Lab. Immunol 9:403-411.
Glurich, I, Grossi, S. Albini, B, Shah, R, Zeid, Z, Baumann, H, and De Nardin, E. 2002. Systemic inflammation in cardiovascular and periodontal disease: a comparative study. Clin. Diagnostic Lab. Immunol 9:425-432
De Nardin, E. 2001. The role of inflammatory and immunological mediators in Periodontitis and CVD. Annals Periodontol. 6:30-40.
Noak, B, Genco, RJ, and De Nardin, E. 2001. Periodontal contribute to elevated systemic C-reactive Protein levels. J. Periodontol. 72:1221-1227.
Noack, B, De Nardin, E, Genco, RJ, and Hoffmann, T. 2001. [Systemic acute phase response in marginal periodontitis] (German). Deutsche Zahnarztliche Zeitschrift, 56:122-125.
Katancik, JA, Sharma, A, and E. De Nardin. 2000. Point mutagenesis studies of the extracellular binding regions of the human CXC receptor-2. Cytokine, 12:1480-1488
Lorton, D., Schaller, J. Lala, A, and De Nardin, E. 2000. Chemotactic-like receptors and Ab peptide induced responses in Alzheimer’s disease. Neurobiol. Aging , 21:463-473.
Miura, Y, Tohyama, Y, Hishita, T, Lala, A, De Nardin, E, Yamamura, H, Uchiyama, T, and K. Tohyama. 2000. Pyk2 and Syk participate in functional activation of granulocytic HL-60 cells in a different manner. Blood, 96:1733-1739.
Guo, M, Han, Y. , Sharma, A., and E. De Nardin. 2000. Characterization of human IgG Fc Receptors of Fusobacterium nucleatum. Oral Microbial Immunol., 15:119-123.
Guo, M., Reynolds, H., Stinson, M., and De Nardin, E. 2000. Isolation and characterization of a human neutrophil aggregation-defective mutant of Fusobacterium nucleatum. FEMS Immunol. Med. Microbiol., 27:241-246.
Nguyen, L.T., Ramanathan, M., Munschauer, F., Brownscheidle, C., Krantz, S., Umhauer, M., Miller, C., De Nardin, E., and L.D. Jacobs. 1999. Flow cytometric analysis of in vitro pro-inflammatory cytokine secretion in peripheral blood from multiple sclerosis patients. J. Clin. Immunol, 19:179-185.
Gwinn, M., A. Sharma, and E. De Nardin, 1999 Single nucleotide polymorphisms (SNPs) of the formyl peptide receptor in localized juvenile periodontitis. J. Periodontol., 70:1194-1201.
Lala, A, Gwinn, M., and E. De Nardin. 1999. Human formyl peptide receptor function: Role of polar residues conserved and non-conserved. Eur. J. Biochem., 264:495-499.
Murphy, T.F., C. Kirkham, E. De Nardin, and S. Sethi. 1999. Analysis of the antigenic structure and human immune response to outer membrane protein CD of Moraxella catarrhalis. Infect. Immun., 67:4578-4585
Genco, RJ, Glurich-Qutaishat, I, Haraszthy, V, Zambon, JJ, and E. De Nardin. 1998. Overview of risk factors for periodontal diseases and impliocation for diabetes and cardiovascular disease. Compendium Dent. Edu., 19:418-423.
Venkatraman, J.T., Rowland, J.A., De Nardin, E., Horvath, P.J. and Pendergast, D. Influence of levels of dietary lipids and excercise (VO2 max) on immune status in runners. Med. Sci. in Sports and Exercise, 29:333-344, 1997.
Katancik, J.A., Sharma, A., Radel, S.J. and De Nardin, E. Mapping of the extracellular binding regions of the human Interleukin-8 type B receptor. Biochem. Biophys. Res. Comm., 232:663-668, 1997.
Lala, A., Sojar, H.T. and De Nardin, E. Expression, purification and immunochemical characterization of recombinant human FMLP receptor. Biochem. Pharmacol., 54:381-390, 1997.
Dentino, A.R., Periathamby, A.R. and De Nardin, E. Subtle differences between human and rabbit neutrophil receptors shown by the secretagogue activity of constrained formyl peptides. Arch. Biochem. Biophys,. 337:267-274, 1997.
De Nardin, E. The molecular basis for neutrophil dysfunction in early onset periodontal diseases. J. Periodontol., 67:345-354, 1996.
Gonzalez, Y., DeNardin, A.M., Grossi, S., Machtei, E., Genco, R.J. and De Nardin, E. Serum cotinine levels and periodontal attachment loss. J. Dental Res., 75:796-802, 1996.
Lala, A., Sojar, H.T. and De Nardin, E. A new quantitative approach for the detection of FMLP receptor proteins in solution using a photoaffinity ligand. Peptide Res., 9:58-60, 1996.
Zheng, W., Kiura, K., Milisauskas, V., De Nardin, E. and Nakamura, I. Murine NK cell allospecificity-1 is defined by inhibitory ligands. J. Immunol., 156:4651-4655, 1996.
Basiri, H., Kiura, K., De Nardin, E. and Nakamura, I. Allospecificities of B6D2F1 hybrid NK cell subsets defined by Ly-49A expression. J. Immunol., 155:2822-2832, 1995.
Brant, E., Sojar, H., Sharma, A., Bedi, G., Genco, R.J. and De Nardin, E. Identification of linear antigenic sites on the Porphyromonas gingivalis 43 kDa fimbrillin subunit. Oral Microbiol. Immunol., 10:146-150, 1995.
Lala, A., Amano, A., Sojar, H.T., Radel, S.J. and De Nardin., E. Porphyromonas gingivalis trypsin-like protease: a possible natural ligand for the neutrophil formyl peptide receptor. Biochem. Biophys. Res. Comm., 199:1489-1496, 1994.
De Nardin, E. Neutrophil receptors: N-formyl-l-methionyl-l-leucyl-l-phenylalanine and Interleukin-8. In: Molecular pathogenesis of periodontal disease, Genco ed., ASM press, Washington DC, pp. 351-361, 1994.
Radel, S.J., Genco, R.J. and De Nardin, E. Structural and functional characterization of the human formyl peptide receptor ligand binding region. Infect. Immun., 62:1726-1732, 1994
Lala, A., Sharma, A., Sojar, H., Radel, S., Genco R. and De Nardin, E. Recombinant Expression and Partial Characterization of the Human Formyl Peptide Receptor. Biochim. Biophys. Acta, 1178:302-306, 1993.
Sorge, K.F., Potts, T.V. and De Nardin, E. Application of PCR to oral diagnostic. In: PCR-Based Diagnostics, Ehrlich and Greenberg editors. Blackwell Scientific Publications, pp. 313-331, 1993.
De Nardin, E., Radel, S.J., Lewis, N., Genco, R.J. and Hammarskjöld, M. Identification of a gene encoding for the human formyl peptide receptor. Biochem. Interl., 26:381-387, 1992.
Radel, S., Genco, R.J. and De Nardin, E. Use of synthetic peptides for the functional mapping of the human formyl peptide receptor. Biochem. Int., 25:745-753.
Gillespie, J., De Nardin, E., Radel, S.J. Kuracina, J., Smutko, J. and Zambon, J. Production of an extracellular toxin by the oral pathogen Wolinella recta. Microb. Pathogenesis, 12:69-77, 1992.
De Nardin, E. and Genco, R.J. Immunochemical characterization of the formyl peptide receptor moieties on human neutrophils. Hybridoma, 10:49-63, 1991.
De Nardin, E. Il ruolo dei neutrofili nelle malattie parodontali. Il Progresso Odontoiatrico, IV 3:15-20, 1991.
De Nardin, E., De Luca, C., Levine, M.J. and Genco, R.J. Antibodies directed to the chemotactic factor receptors detect differences between chemotactically normal and defective neutrophils from LJP patients. J. Periodontol., 61:609-617, 1990.