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DAB Substrate Kit (HRP-based immunostaining)

Components:

DAB Substrate: 1 ml, store at room temperature
Stabilized Peroxide: 200 ml, store at room temperature

Product Description: One of the most widely used enzymes for chromogenic detection in immunoassays is horseradish peroxidase (HRP), a
highly stable enzyme (Clin. Chim. Acta 81:1,1977). The compound 3,3'-diaminobenzidine (DAB) is a highly sensitive HRP substrate and is thus
widely used in immunohistological and immunoblotting assays (J Histochem Cytochem 36, 317, 1988). HRP in the presence of peroxide converts
DAB to a brownish water-insoluble polymeric product, which can then be digitally imaged. The working substrate solution needs to be prepared
fresh by mixing DAB Substrate solution and Peroxide solution. The Western Blotting Substrate kit is stable for ~1 year if stored and handled
properly.