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Tyrosine Aminotransferase (TAT) Assay Kit

Components:

TAT Substrate: 5 ml; store at -80°C

TA Assay Solution: 10 ml; store at -80°C (for 200 wells)

10X Cell Lysis Solution: 25 ml; store at 4°C

Product Description: Tyrosine aminotransferase (TAT) is the first enzyme of a hepatic pathway involved in tyrosine degradation via
homogentisate, catalyzing the following reaction: L-tyrosine + alpha-ketoglutarate <-> 4-hydroxyphenylpyruvate + L-glutamate. The TAT gene is
regulated by glucocorticoid hormones and cAMP, and alterations in catalytic activity of the enzyme usually parallel changes in enzyme amount.
Tyrosinemia (type II or Richner-Hanhart syndrome, RHS) is the most common metabolic disease caused by TAT mutations (more than 10
identified). RHS patients exhibit elevated blood levels of tyrosine and hydroxyphenylpyruvate accompanied by mental retardation and keratitis
(deposition of tyrosine crystals in the cornea). The TAT activity assay is based on sequential transamination reaction and glutamate
dehydrogenase reaction, which couples the reduction of INT to INT-formazan (molar extinction coefficient = 18/mM-cm at 492 nm), allowing for
sensitive detection of TAT enzyme activity in plasma and cell/tissue samples. Assay solutions are stable for several years if stored and handled
properly.