Our websites: www.acsu.buffalo.edu/~chunglee or www.bmrservice.com

            Senescent Cell Detection Kit                                                                       

K         Kit Components:

            SCD Buffer: 150 ml, store at 4ºC
            Ferricyanide: 10 ml, store at 4ºC
            Ferrocyanide: 10 ml, store at 4ºC
            X-gal: 4 ml, store at -20ºC

            Product Description: Cellular senescence plays a critical in tumor suppression. Normal mitotic somatic cells gradually cease division after
            continuous expansion in culture and enter a state referred to as replicative aging or senescence, exhibiting a Hayflick limit of ~50 population
            doublings (Exp Cell Res 37:614, 1965). It has been suggested that various changes associated with cellular aging may be closely mediated by
            actin cytoskeleton organization and focal adhesions among cells and extracellular matrix. Senescent cells typically exhibit an enlarged flattened
            “frying egg” morphology associated with increased activity of acidic beta-galactosidase (J Cell Sci 113:3613, 2000). The increased activity of
            lysosomal acid beta-galactosidase is usually detected at pH 6, and constitutes the basis of the Senescent Cell Detection assay using the
            histochemical substrate 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (Xgal) for in situ cell staining. The assay kit is sufficient for 300 - 500
            assays. Note that the fixative is not included in the kit.