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Sorbitol Dehydrogenase (SDH) Assay Kit

Components:

SDH Assay Solution: 10 ml; store at -80°C (for 200 wells)

50x SDH Substrate: 0.25 ml; store at -80°C

10X Cell Lysis Solution: 25 ml; store at 4°C

Product Description: Sorbitol dehydrogenase (SDH), found in organisms from bacteria to humans, converts sorbitol, the sugar alcohol form of glucose, to fructose, and the zinc-dependent reaction uses NAD+ as a cofactor. Organs abundant in SDH activity include the liver, kidney, lens and seminal vesicle. SDH and aldose reductase (AR) form the polyol pathway that interconverts glucose and fructose. Since SDH activity promotes the formation of NADH, the redox change induced by elevated SDH may have a pathogenic role in certain disease state, making SDH a potential therapeutic target. In addition, SDH has a close evolutionary relationship with alcohol dehydrogenase (ADH). Our non-radioactive colorimetric SDH
assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased SDH activity. The kit is stable for at least one year under proper storage and handling conditions. The assay is simple, non-radioactive, and very sensitive. Each kit is sufficient for 200 microassays.