Our websites: www.acsu.buffalo.edu/~chunglee or www.bmrservice.com

Whole Blood Genomic DNA Isolation Kit

Components: 

TE:       TE:    200 ml, store at 4°C (for ~100 samples)

Product Description: Our Whole Blood Genomic DNA Isolation Kit uses a method that does not involve extensive DNA purification steps and yet yields clean genomic DNA from whole blood, clinical swabs, white blood cells, and tissue culture cells that can be used for PCR amplification. The kit is ideally suited for any situation where cells are readily collected by soft abrasion. Clinical samples collected with cotton swabs can be stored in TE prior to DNA isolation and PCR analysis. The protocol is derived from the method based on digestion of cells with Proteinase K in the presence of SDS and EDTA (Eur. J. Biochem. 36:32,1973). We have modified and streamlined the method, allowing genomic DNA to be isolated with minimal steps. Reagents are stable for at least one year if handled and stored properly. The product is for research use only.

Features: Genomic DNA can be rapidly isolated in 60 min without phenol extraction and column elution steps. See Standard Genomic DNA Isolation Kit. The method is adaptable to automation.