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Pyruvate Kinase Assay Kit

Components:

PK Reaction Solution- 0.5 ml, store at -80ºC
1 mM EDTA- 10 ml, store at 4ºC
10x Cell Lysis Solution- 25 ml, store at 4ºC

ATP Assay Solution: 10 ml, store at -80ºC

1 mM ATP Standard: 0.5 ml, store at -80ºC

Product Description: Pyruvate kinase (PK) catalyzes the last step of aerobic glycolysis, converting phosphoenolpyruvate (PEP) to pyruvate with production of one ATP. Liver PK is subject to hormonal and allosteric regulation, which is involved in maintaining blood glucose homeostasis. The importance of this substrate-level phosphorylation step is underscored by the finding that PK deficiency, one of the most common enzymopathies, manifests clinically as hemolytic anemia. The clinical severity of PK deficiency varies widely, ranging from a mildly compensated anemia to severe childhood anemia. In addition, PK isoform switch is known to promote tumorigenesis. The assay kit can measure PK activity present in crude
cell/tissue extracts. Luciferase-based detection of ATP allows fast, sensitive, and quantitative assay of PK. Kit components are stable for many years if stored and handled properly. The product is for research and clinical diagnostic applications.