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Nucleoside Diphosphate Kinase (NDK) Assay Kit

Components:

NDK Reaction Solution- 0.4 ml, store at -80ºC

NDK Control Solution- 0.4 ml, store at -80ºC
4 mM EDTA- 10 ml, store at 4ºC
10x Cell Lysis Solution- 25 ml, store at 4ºC

ATP Assay Solution: 10 ml, store at -80ºC

1 mM ATP Standard: 0.5 ml, store at -80ºC

Product Description: Nucleoside-diphosphate kinase (NDK) catalyzes the exchange of phosphate groups between different nucleoside
diphosphates with little specificity for the base and the sugar: ATP + NDP  ADP + NTP. Thus, nucleotide analogues used in antiviral therapy are
also phosphorylated by NDK to their triphosphate forms. In energy metabolism, NDK serves to maintain an equilibrium between the concentrations
of different nucleoside triphosphates. For example, GTP produced in the Krebs or TCA cycle is readily converted to ATP through the mediation of
NDK, and the enzyme has a major role in the synthesis of nucleoside triphosphates other than ATP. There are two isoforms of NDK in higher
eukaryotes: NDK-A and NDK-B, which have similar structure and function, and can combine in any proportion to form functional NDK hexamers.
The enzyme activity assay kit can measure NDK activity present in serum/plasma and crude cell/tissue extracts. Luciferase-based detection of
ATP produced by NDK reaction allows fast, sensitive and quantitative assay of NDK activity. Measurement of ATP requires a luminometer or
scintillation counter. Kit components are stable for many years if stored and handled properly.