Our websites: www.acsu.buffalo.edu/~chunglee or www.bmrservice.com

Mini-Plasmid DNA Purification Kit

Components:        

Plasmid Solution-1 (PS-1): 30 ml, store at 4ºC (sufficient for 300 mini-preps)

Plasmid Solution-2 (PS-2): 30 ml, store at room temperature

Plasmid Solution-3 (PS-3): 30 ml, store at 4ºC

Plasmid Solution-4 (PS-4): 6 ml, store at 4ºC

Product Description: Isolation of high-quality plasmid DNA from the gemisch of the E. coli cell lysate paves the way for many laboratory as well as clinical genetic applications, among which are clone screening, DNA sequencing, DNA transfection, probe preparation, PCR analysis, and gene therapy, to name but a few. Small-scale plasmid purification (mini-prep) is often used to facilitate clone screening and DNA sequencing. Although many commercial mini-prep kits are offered, few combines the following three features: simplicity, low cost, and high quality. We have modified and streamlined the alkaline lysis mini-prep method of Sambrook et al. (Molecular Cloning, CSH, NY) eliminating the needs for additional RNase treatment and phenol-chloroform extraction. Plasmid DNA can be easily and rapidly purified from an overnight E. coli culture in 15 min with a typical yield of 1 – 5 mg DNA per ml of culture. The DNA is suitable for restriction digestion, probe preparation, and PCR analysis. DNA to be used for sequencing analysis is subjected to an additional 10-min step of PEG precipitation (PS-4) to remove any trace amount of RNA. High-quality DNA sequencing ladders can be easily obtained (almost 1,000 bases resolved per sequencing run) if the plasmid DNA is isolated from a suitable E. coli host strain. The kit (sufficient for 300 mini-preps) is stable for at least one year if handled and stored properly. The product is for research use only.

Features: The Mini-Plasmid DNA Isolation Kit allows the user to obtain 1 – 5 mg plasmid DNA per ml of culture in 15 min without the use of phenol. Isopropanol, 70% ethanol, and sterilized distilled water are not included in the kit. The only equipment you need is a bench-top centrifuge. See Maxi-Plasmid DNA Purification Kit. The method is adaptable to automation.