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Malate Dehydrogenase (MDH) Assay Kit

Components:

MDH Assay Solution: 10 ml; store at -20°C (for 200 microassays)

10X Cell Lysis Solution: 25 ml; store at 4°C

Product Description: Mitochondrial malate dehydrogenase (MDH2) catalyzes the last reaction of the TCA or Krebs cycle, converting malate and NAD+ to oxaloacetate and NADH in the mitochondria. In reversing the reaction, MDH is also involved in gluconeogenesis, allowing oxaloacetate/malate to leave the mitochondria. Once in the cytosol, the malate is oxidized back to oxaloacetate by cytosolic MDH or MDH1, which is followed by conversion of oxaloacetate to phosphoenolpyruvate by phosphoenolpyruvate carboxykinase (PEPCK). The interconversion between malate and oxaloacetate also constitutes the essential steps of the malate-aspartate shuttle. MDH exists in most organisms as a homodimeric molecule with subunits weighing between 30 and 35 kDa. The MDH enzyme activity assay kit can be used to quickly monitor potential changes in the activity of MDH during various cellular processes and mitochondrial biogenesis. The non-radioactive colorimetric MDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased MDH activity. The kit is stable for at least one year if handled and stored properly. The product is for research use only.

Features: The superior sensitivity of the non-radioactive assay kit allows the assay to be completed within 20-30 min with minimal pipetting steps. Each kit is sufficient for approximately 200 assays using the 96-well format. See related product LDH assay kit, GAPDH assay kit, G6PD assay kit, PDH assay kit, Glucose assay kit, and Lactate Assay Kit. The method is adaptable to automation.