Our websites: www.acsu.buffalo.edu/~chunglee or www.bmrservice.com
Low-Range DNA Markers
Components:
Low-Range DNA Marker Solution: 0.4 ml (200 lanes); store at 4ºC
Product Description:
The
Low-Range DNA Marker set includes 8 linear DNA fragments (from 4.5 Kb to 0.1 Kb)
ready for agarose or polyacrylamide gel analysis. The markers are supplied in a
stabilized loading solution containing sucrose, bromophenol blue (lower dye),
xylene cyanol (upper dye), and EDTA. These DNA fragments typically migrate as
two easily recognizable quadruplets providing a convenient reference. The upper
cluster includes 4 fragments of 4.5 Kb, 1.9 Kb, 1.2 Kb, and 0.8 Kb in sizes. The
lower cluster also includes 4 fragments: 0.42 Kb, 0.29 Kb, 0.21 Kb, and 0.1 Kb.
Each of the 8 DNA fragments carries 5’-overhangs and therefore can be
conveniently radiolabeled by either Klenow (using labeled dATP) or
Polynucleotide Kinase. An additional 60-bp fragment becomes visible when the
marker set is overloaded or labeled with an isotope. The DNA markers set should
be kept at 4ºC. The product is for research only.
Upper quadruplet:
Lower
quadruplet:
4.5 Kb
0.42
Kb
1.9 Kb
0.29
Kb
1.2 Kb
0.21
Kb
0.8 Kb
0.10
Kb