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Lactate Dehydrogenase (LDH) Staining Kit

Components:

LDH Staining Solution: 15 ml; store at -80°C

10X Cell Lysis Solution: 25 ml; store at 4°C

Product Description: Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. It consists of 4 subunits which may be of 2 different types: M (muscle) and H (heart), also known as A and B respectively. Five different isoenzymes are therefore possible, depending on the subunit composition. The LDH staining kit is based on the reduction of a tetrazolium salt to a formazan product, which is water insoluble and exhibits an intense dark blue color. Since the intensity of the dark blue color is proportional to LDH activity, the staining kit can be used to delineate cell/tissue LDH isoform activity by either gel assay or in situ staining.