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Glutathione S-Transferase (GST)

Product Description: Many recombinant proteins are expressed as GST-fusion proteins to facilitate purification ¹ . The recombinant GST protein is encoded by the parasitic Schistosoma japonicum glutathione S-transferase gene. The parasite GST protein shows ~60% homogy with class Mu isozymes of mammalian GST (EC 2.5.1.18) ² . The mammalian GST super family consists of four classes: Alpha, Mu, Pi, and Theta. GST catalyzes the conjugation of reduced glutathione to electrophiles on many substrates, thus detoxifying endogenous compounds such as lipid peroxides. The recombinant GST can be used as a negative control for recombinant growth factors expressed as GST fusion proteins. The C-terminal end of the protein harbors an engineered thrombin cleavage site and a protein kinase A (PKA) phosphorylation site. The protein can therefore be radioactively labeled with 32P-g-ATP and PKA. The product is for research use only.