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Glutaminase (GLS) Assay Kit

Components:

Glutaminase Buffer: 10 ml; store at 4°C

Glutamine: 50 mg; store at 4°C

TA Assay Solution: 10 ml (200 wells); store at -80°C

10X Cell Lysis Solution: 25 ml; store at 4°C

Product Description: Glutaminase (glutamine aminohydrolase or GLS) catalyzes the following reaction: Glutamine + H₂O → Glutamate + NH_3.  The enzyme has tissue-specific roles in multiple organs. Two different mammalian phosphate-activated GLS isoforms are known: GLS1 (kidney-type) and GLS2 (liver-type; a target of the tumor suppressor protein p53). The hydrolytic activity of glutaminase generates ammonia for urea synthesis in the liver similar to that mediated by glutamate dehydrogenase. During renal acidosis, glutaminase is induced in the kidney, leading to increased excretion of ammonia, which plays an important role in maintaining acid-base homeostasis. Glutaminase regulates the levels of the neurotransmitter glutamate in the brain. The rate of glutaminolysis is known to increase in tumors and may be a hot spot for regulation of cancer cell metabolism. Inhibitors of GLS may therefore be candidate drugs for cancer therapy. The GLS assay is based on sequential GLS-mediated hydrolytic reaction and glutamate dehydrogenase reaction, which couples the reduction of INT to INT-formazan (e = 18 mM^-1cm^-1 at 492 nm), allowing for sensitive detection of GLS enzyme activity in plasma and cell/tissue samples. Assay solution is stable for several years if stored and handled properly.