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Human FGF-2

        Product Description: Fibroblast growth factor-2 (FGF-2 or b-FGF) belongs to the FGF family of more than 20 growth factors possessing broad mitogenic and angiogenic activities through differential interactions with four major tyrosine kinase FGF receptors. Cross-talk mechanisms exist among FGF-2 and different members of the VEGF family during angiogenesis and vasculogenesis. This property has implicated FGF-2 in diverse areas of preclinical studies such as tumor growth, vascular and bone remodeling, and cardiac hypertrophy. Multiple forms of FGF-2 are generated by alternative translation on the same mRNA transcript, producing a low molecular weight (LMW ~18 kDa) and several high molecular weight (HMW 20-24 kDa) forms from AUG (methionine) and CUG (leucine) codons, respectively. All FGF-2 forms lack a classical signal peptide that directs secretion through the endoplasmic reticulum-Golgi pathway. The recombinant FGF2 is expressed as a GST-fusion protein. The fusion junction of the protein harbors an engineered thrombin cleavage site and a protein kinase A (PKA) phosphorylation site. The GST portion of the fusion protein can be cleaved off by thrombin if necessary. The fusion protein can be radioactively labeled with 32P-g-ATP and PKA. The product is for research use only.