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TMB Substrate Kit (HRP-based ELISA)

Components:

TMB Substrate: 16 ml, store at room temperature

Stabilized Peroxide: 200 ml, store at room temperature

Product Description: One of the most widely used enzymes for chromogenic detection in immunoassays is horseradish peroxidase (HRP), a highly stable enzyme (Clin. Chim. Acta 81:1,1977). The compound 3,3',5,5'-tetramethylbenzidine (TMB) has emerged as the safest colorigenic substrate of HRP exhibiting excellent sensitivity (Tetrahedron 30:3299,1974). HRP in the presence of peroxide converts TMB to a bluish reaction product that can be read spectrophotometrically at 370 or 655 nm. The blue end product upon acidification by HCl or H2SO4 generates a bright yellowish color enabling sensitive HRP detection and quantification by absorbance at 450 nm. This application has become widely used in enzyme-linked immunosorbent assay (ELISA) because of its lowest detection limit compared to other reporter enzymes and substrate systems. The working substrate solution needs to be prepared fresh by mixing TMB Substrate solution and TMB Peroxide solution. The ELISA Detection kit is stable for ~1 year if stored and handled properly.The product is for research use only.