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Dipeptidyl peptidase-4 (DPP-4 or CD26) Assay Kit

Components:

 DPP-4 Assay Buffer: 5 ml; store at 4°C (for 100 assays)

10x Cell Lysis Solution: 25 ml; store at 4°C

100x Gly-Pro-pNA: 0.05 ml, store at -20°C (for 100 assays)

Product Description: Dipeptidyl peptidase-4 (DPP-4; CD26) is a serine exopeptidase expressed on the surface of most cell types, capable of
cleaving X-proline or X-alanine dipeptides from the N-terminus of polypeptides. There are more than 60 identified protein substrates of DPP-4,
including chemokines, growth factors, neuropeptides and vasoactive peptides. The pleiotropic effects of DPP-4 regulate glucose metabolism,
inflammation, appetite, immune function and extracellular matrix remodeling. Notably, inhibitors of DPP-4 have emerged as a new class of
antidiabetic drugs through stabilization of two key glucose-regulating hormones: glucose-dependent insulinotropic polypeptide (GIP) and
glucagon-like peptide-1 (GLP-1). The DPP-4 activity assay kit is based on proteolytic hydrolysis of the chromogenic dipeptide substrate
Gly-Pro-p-nitroanilide hydrochloride (Gly-Pro-pNA) under an optimized condition. Cleavage of the pNA from the dipeptide increases absorbance at
405 nm (extinction coefficient= 9.5/mM-cm) and 410 nm (extinction coefficient= 8.8/mM-cm), allowing for sensitive and quantitative assay of DPP-4
activity present in tissue/cell lysates and biological fluids. Kit components are stable for ~1 year if stored and handled properly.