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D-Lactate Assay Kit

 

Components:

 

            D-Lactate Assay Solution: 10 ml; store at -20°C or -80°C (200 assays)

1 mM D-Lactate Standard: 0.5 ml; store at -20°C

PEG Solution: 15 ml; store at 4°C

 

Product Description: D-lactate accumulation in body fluids indicates bacterial infection or metabolic abnormality, and detection of D-lactate is therefore of clinical value. Unlike L-lactate, which is present at mM levels in the circulation, D-lactate is usually too low to be detected in healthy subjects. The D-Lactate Assay Kit is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which exhibits an absorption maximum at 492 nm. The D-Lactate Assay kit can measure the concentration of D-lactate present in serum or plasma with a detection limit of ~10 mM. Samples need to be deproteinized with TCA prior to assay. Assay solution is stable for at least one year if stored properly. Keep assay solution at -80ºC for long term storage (more than 1 months). The product is for research use only.

 

Features: The non-radioactive assay kit allows the assay to be completed in one step within 30 min. The kit can be used for clinical samples such as serum and plasma. Each kit is sufficient for 200 assays using the 96-well format. The assay can be scaled up for spectrophotometric measurements. The only equipment required is a spectrophotometer or a microplate reader.