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Catalase Assay Kit

Kit Components:

            Catalase Substrate Solution: 25 ml (250 microassays), store at 4ºC

            Catalase Assay Solution: 25 ml, store at 4ºC

            10x Cell Lysis Solution: 25 ml, store at 4ºC

Product Description: Catalase, present in nearly all aerobic cells, serves to protect the cell against oxidative damage caused by hydrogen peroxide (H2O2). Hydrogen peroxide, although not a free radical, is highly reactive. Catalase catalyzes its decomposition into molecular oxygen and water without the production of free radicals. Catalase also uses hydrogen peroxide to oxidize toxins including phenols, formic acid, formaldehyde, and alcohols. The beef liver catalase monomer consists of 506 amino acids, a heme prosthetic group, and one NADH molecule. The enzyme is among the most efficient known, with rates approaching 200,000 catalytic events/second/subunit, which is near the diffusion-controlled limit. Our catalase assay system is based on the ability of hydrogen peroxide to oxidize an azo chromogen with an accompanying increase in O.D.410-420 nm, which can be conveniently quantified by spectroscopy. The presence of catalase reduces the amount of hydrogen peroxide, thus inhibiting the oxidation of the azo chromogen and the appearance of the yellowish color. This inhibition of chromogen oxidation is thus proportional to the activity of catalase present in the sample. As little as 10³-10⁴ cells can be used per assay. The product is stable for at least 1 year if stored and handled properly, and is for research use only.

Features: The assay is simple, non-radioactive, and very sensitive. Each kit is sufficient for 250 microassays.