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BCIP Substrate Kit (for ALP-based immunostaining)

Components:

20x ALP Buffer: 10 ml, store at room temperature

50x BCIP: 4 ml, store at -20°C

50x Tetrazolium: 4 ml, store at -20°C

Product Description: Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) are two reporter enzymes widely used in immunoassays due to their stability. 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) is a synthetic chromogenic substrate, which upon cleavage by alkaline phosphatase is converted to 5-bromo-4-chloro-3-indole and inorganic phosphate. In the presence of a tetrazolium salt such as NBT or MTT, 5-bromo-4-chloro-3-indole is further converted to an insoluble dark blue diformazan precipitate, thus enabling its use for sensitive ALP-based protein detection. In addition, the kit provides an easy and sensitive method for detecting the endogenous ALP activity, which may be differentially regulated during development and differentiation. The working solution can be used for ALP-based in situ immunostaining and Western blotting. The kit is sufficient for preparing 200 ml of ALP staining solution.