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Adenylate Kinase Assay Kit

Components:

AK Reaction Solution- 0.5 ml, store at -80ºC
2 mM EDTA- 10 ml, store at 4ºC
10x Cell Lysis Solution- 25 ml, store at 4ºC
ATP Assay Solution- 10 ml (100 assays), store at -80ºC
1 mM ATP Standard- 0.5 ml, store at -80ºC

Product Description: Adenylate kinase (AK or myokinase) catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP (ATP + AMP <=> 2 ADP), i.e., the enzyme relays adenine nucleotide signals to metabolic sensors. This ubiquitous enzyme is thus involved in energy metabolism and nucleotide synthesis. Two AK isozymes have been identified in mammalian cells: AK1 is cytosolic and AK2 is mitochondrial. AK1 deficiency in the erythrocyte is associated with hemolytic anemia. AK1 knockout disrupts the balance between ATP consumption and ATP synthesis and energy homeostasis in the post-ischemic heart, precipitating myocardial ischemia-reperfusion injury. AK2 deficiency in humans causes hematopoietic defects. If abundant ADP is present as the substrate, the AK-catalyzed reaction is driven toward formation of ATP, which provides a linear production of ATP and can be used for measuring AK activity in crude extracts using the luciferase/luciferin system. Luciferase-based detection of ATP produced by AK reaction allows fast, sensitive, and quantitative assay of AK activity. Measurement of ATP requires a luminometer or scintillation counter. Kit components are stable for many years if stored and handled properly.