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ATP/ADP/AMP Assay Kit

Components:

ATP Assay Solution: 2 x 10 ml, store at -80°C (for 200 assays)
1 mM ATP Standard: 0.5 ml, store at -80°C
ADP-CB: 0.5 ml, store at -80°C
AMP-CB: 0.5 ml, store at -80°C
EDB: 4 ml, store at -80°C
ADP-CE: 40 ml, store at 4°C (do not freeze)
AMP-CE: 20 ml, store at 4°C (do not freeze)
4 mM EDTA: 10 ml, store at 4°C

Product Description: Many metabolic reactions are controlled by the energy status of the cell. One index of the energy status is the energy charge
(EC) of the cell, which is determined by the concentrations of adenine nucleotides. Although EC could theoretically range in values from 0 to 1, most
cells maintain a steady-state EC in the range of 0.8 to 0.95, meaning that the energy status of the cell is buffered. ATP, ADP and AMP are also
allosteric effectors for many enzymes. Intracellular adenine nucleotide concentrations vary during cell growth and cell death. Extracellular adenine
nucleotides can regulate various signaling pathways. The assay kit is based on the luciferase reaction in the presence of ATP, which generates
yellow-green light that can be conveniently measured by a luminometer or scintillation counter (Anal. Biochem. 80:496,1977). This provides the
basis for a sensitive ATP assay system capable of detecting 0.1 mM or 1 picomole of ATP. The ATP/ADP/AMP assay kit is sufficient for 200 assays
using 0.1 ml of ATP Assay Solution per assay. Sample ADP and AMP are converted to ATP by pyruvate kinase and adenylate kinase reactions. The
ATP Assay Solution if stored in aliquots at -80°C is stable for many years. Repeated freeze-thaw cycles should be avoided.