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Protein Assay Kit (EDTA/DTT/Mg++ compatible)

Components:        

Protein Dye Solution: 200 ml, store at 4ºC (linear assay range 20 - 200 mg/ml )

100 mg/ml Sterilized Bovine Serum Albumin (BSA): 1 ml, store at -20ºC

Product Description: The protein assay system is formulated from a refined method of Bradford (Anal. Biochem. 72:248,1976), and is designed to streamline the procedure for determining concentration of soluble proteins. This method is based on the binding of proteins to Coomassie Brilliant Blue in an acidic solution resulting in a protein concentration-dependent increase in the absorbance at 595 nm (Anal. Biochem. 64:509,1975), which can be measured with a standard laboratory spectrophotometer or microplate reader. The product is for research use only.

Features: Unlike those used in other commercial protein assay kits, our 595 nm Protein Assay System does not require dilution or filtration of the assay reagent prior to the assay, thus saving time and enhancing assay consistency. Simply mix your protein sample with 20 parts of the assay solution, and relative protein concentration can be immediately obtained by comparison to a standard curve performed at the same time. The 595 nm Protein Assay System has a linear working range of 20 - 200 mg/ml proteins.