The following are instructions for use of the Kinemage software that is available on the computers in H131 or by download off of the URL given on the class homepage. The files used in the class to are available on the computer in H131 or in a WinZip archive at the URL http://www.acsu.buffalo.edu/~koudelka/kinemage.zip.

  1. Open the MAGE program by following the instructions given in the URL

http://www.acsu.buffalo.edu/~koudelka/kinemage.htm.

2. The screen is composed of several areas of information.

a. To the left is a white window with text and it is titled _TEXT_. You can click anywhere on the screen to bring it to the forefront of the screen. Click on it to try this. Now you can reduce the text window size to better view the remainder of the screen. To do this move the cursor over the right edge of the text window until you see a double arrow, click and drag the screen closed enough to view the kinemage better.

b. To the bottom of the screen is another white screen and it is titled _CAPTION_. You can also change the size of this window with the same procedure. For both windows you can also change the horizontal size by the same method. Arrange both windows so that maximize the visibility of the structure window.

3. Determining the function of _BUTTONS_ and _SCROLL BARS_.

To the right of the screen is a list of _BUTTONS_ and some _SCROLL BARS_. Click on and off each of the dominant buttons and notice what changes in the kinemage. Remember that repressor dimer is composed of two sets of chains and the DNA is a third. Each dominant button controls the view of those chains. The last dominant button.

The _SCROLL BARS_ to the right control functions regarding the entire image.

1. _ZOOM_ allows you to move in or out for selected areas of viewing

2. _Z SLAB_ and _Z TRAN_ allows you to control the depth of field that you see.

4. Moving the kinemage

MAGE makes it possible to rotate the image in any of the three dimensions. This can be accomplished one dimension at a time or a composition of three at once.

a) Place the arrow (your cursor pointer when on the kinemage) at the extreme top center of the kinemage. Click and hold the mouse. Notice the part of the arrow change to a white box with a black dot. While holding the mouse button down move left or right. This function allows you to rotate the kinemage in one dimension. Notice you can only go so far before the motion is reversed.

b) Place the arrow on the extreme left or right center. This time when you click and hold you should see a plus sign on the arrow. Move the mouse straight up or down and notice the dimensional move.

c) If you move the cursor to any other position on the kinemage and click the arrow again is present. You can move in multidimensional directions by controlling the mouse movement.

5. The Menu Bar

a. If you wish to return the kinemage to the original position there is a function on the

menu bar to do so. Locate the _VIEWS_ (or OTHER if in H131) title on the menu bar above the kinemage. Click and select view 1. Notice that the view returns to the original position.

b. Under the _DISPLAY_ menu there are three options for line width on the kinemage. Click each one and notice that the line thickness changes.