Research Group -----------Ann-----------Nicole and Jesse -------------Gym Buddies------------The Bleeding Gumz Research Profile Our laboratory is currently
investigating the mechanisms by which various inflammatory and immune
molecules
and their respective receptors activate and regulate the immune
response to
various pathogenic microorganisms. In particular, we are investigating
various
genetic polymorphisms in the genes which encode for these molecules,
and how
these polymorphism may affect their normal functions and/or are
associated with
susceptibility to disease. Molecules under study include various
inflammatory
cytokines and their receptors as well as fibrinogen and other acute
phase
proteins. Our laboratory is also studying the function of neutrophils,
which
are primary phagocytic cells that play a key role in host defense
against
extracellular bacteria, as well as participate in the acute phase of
inflammatory reactions. Funding for research is provided by grants from
the
National Institute of Health. Representative publications Boehm, T.K., E. De Nardin. Fibrinogen binds IgG antibody and enhances IgG-mediated phagocytosis. 2008. Hum. Antibodies (in press). Sahingur, E, T.K. Boehm, H.T. Sojar, A. Sharma, and E. De Nardin. 2006 Fibrinogen-Neutrophil Interactions in response to fMLP and Porphyromonas gingivalis Fimbrial peptides. Immunol. Invest. 35:63-74. Hajishengallis G, Sojar H, Genco RJ, and De Nardin E. 2004. Intracellular signaling and cytokine induction upon interactions of Porphyromonas gingivalis fimbria with pattern-recognition receptors. Immunol. Invest. 33, 1–16. Yamamoto K, Kobayashi T, Ho A, Genco RJ,
Yoshie H, and De Nardin E. 2004. Association of Fcg Receptor IIa
Genotype with Chronic
Periodontitis in Caucasians. J.
Periodontol., 75:517-522. Sahingur, SE, Genco, RJ, Sharma, A, and De Nardin, E. 2003. Association of Increased Levels of Fibrinogen and the –455G/A Fibrinogen Gene Polymorphism with Chronic Periodontitis. J. Periodontol. 74:329-337. Hajishengallis G, Martin M, Hakimuddin S, Sharma A, Schifferle RE, Denardin E, Genco RJ. 2002. Dependence of bacterial protein adhesions on toll-like receptors for proinflammatory cytokine induction. Clin. Diagnostic Lab. Immunol 9:403-411. Glurich, I, Grossi, S. Albini, B, Shah, R, Zeid, Z, Baumann, H, and De Nardin, E. 2002. Systemic inflammation in cardiovascular and periodontal disease: a comparative study. Clin. Diagnostic Lab. Immunol 9:425-432 De Nardin, E. 2001. The role of inflammatory and immunological mediators in Periodontitis and CVD. Annals Periodontol. 6:30-40. Noak, B, Genco, RJ, and De Nardin, E. 2001.
Periodontal
contribute to elevated systemic C-reactive Protein levels.
J.
Periodontol. 72:1221-1227. Noack,
B, De Nardin, E, Genco, RJ, and Hoffmann, T. 2001. [Systemic acute
phase
response in marginal periodontitis] (German). Deutsche
Zahnarztliche Zeitschrift, 56:122-125. Katancik, JA, Sharma, A, and E. De Nardin. 2000.
Point
mutagenesis studies of the extracellular binding regions of the human
CXC
receptor-2. Cytokine, 12:1480-1488 Lorton, D., Schaller, J. Lala, A, and De Nardin, E. 2000. Chemotactic-like receptors and Ab peptide induced responses in Alzheimer’s disease. Neurobiol. Aging , 21:463-473. Miura, Y, Tohyama, Y, Hishita, T, Lala, A, De
Nardin, E,
Yamamura, H, Uchiyama, T, and K. Tohyama. 2000. Pyk2 and Syk
participate in
functional activation of granulocytic HL-60 cells in a different
manner. Blood, 96:1733-1739. Guo, M, Han, Y. , Sharma, A., and E. De Nardin.
2000.
Characterization of human IgG Fc
Receptors of Fusobacterium nucleatum. Oral
Microbial Immunol., 15:119-123. Guo, M., Reynolds, H., Stinson, M., and De
Nardin, E. 2000. Isolation
and characterization of a human neutrophil
aggregation-defective mutant of Fusobacterium nucleatum. FEMS Immunol. Med.
Microbiol., 27:241-246. Nguyen, L.T., Ramanathan, M., Munschauer, F.,
Brownscheidle, C., Krantz, S., Umhauer, M., Miller, C., De Nardin, E.,
and L.D.
Jacobs. 1999. Flow cytometric analysis of in
vitro pro-inflammatory cytokine
secretion in peripheral blood from multiple sclerosis patients. J. Clin.
Immunol, 19:179-185. Gwinn, M., A. Sharma, and E. De
Nardin, 1999 Single
nucleotide polymorphisms (SNPs) of the formyl peptide receptor in
localized
juvenile periodontitis. J. Periodontol.,
70:1194-1201. Lala, A, Gwinn, M., and E. De Nardin.
1999. Human formyl peptide receptor function: Role of polar
residues conserved and non-conserved. Eur.
J. Biochem., 264:495-499. Murphy, T.F., C. Kirkham, E. De Nardin, and S.
Sethi. 1999. Analysis
of the antigenic structure and human immune
response to outer
membrane protein CD of Moraxella
catarrhalis. Infect. Immun.,
67:4578-4585 Genco, RJ, Glurich-Qutaishat, I, Haraszthy, V,
Zambon, JJ,
and E. De Nardin. 1998. Overview of risk factors for periodontal
diseases and
impliocation for diabetes and cardiovascular disease.
Compendium
Dent. Edu., 19:418-423. Venkatraman, J.T., Rowland, J.A., De Nardin, E.,
Horvath,
P.J. and Pendergast, D. Influence of
levels of dietary lipids and excercise (VO2 max) on immune status in
runners. Med. Sci. in Sports
and Exercise, 29:333-344, 1997. Katancik, J.A., Sharma, A., Radel, S.J. and De
Nardin,
E. Mapping of the extracellular binding
regions of the human Interleukin-8 type B receptor.
Biochem.
Biophys. Res. Comm., 232:663-668,
1997. Lala, A., Sojar, H.T. and De Nardin, E. Expression, purification and
immunochemical
characterization of recombinant human FMLP receptor.
Biochem.
Pharmacol., 54:381-390, 1997. Dentino, A.R., Periathamby, A.R. and De Nardin,
E. Subtle differences between human
and rabbit
neutrophil receptors shown by the secretagogue activity of constrained
formyl
peptides. Arch. Biochem. Biophys,. 337:267-274, 1997. De Nardin, E. The
molecular basis for neutrophil dysfunction in early onset periodontal
diseases. J. Periodontol.,
67:345-354, 1996. Gonzalez, Y., DeNardin, A.M., Grossi, S.,
Machtei, E.,
Genco, R.J. and De Nardin, E. Serum
cotinine levels and periodontal attachment loss. J. Dental Res., 75:796-802,
1996. Lala, A., Sojar, H.T. and De Nardin, E. A new quantitative approach for the detection
of FMLP receptor proteins in solution using a photoaffinity ligand. Peptide
Res., 9:58-60, 1996. Zheng, W., Kiura, K., Milisauskas, V., De
Nardin, E. and
Nakamura, I. Murine NK cell
allospecificity-1 is defined by inhibitory ligands.
J.
Immunol., 156:4651-4655, 1996. Basiri, H., Kiura, K., De Nardin, E. and
Nakamura, I. Allospecificities of B6D2F1
hybrid NK cell
subsets defined by Ly-49A expression. J. Immunol., 155:2822-2832, 1995. Brant, E., Sojar, H., Sharma, A., Bedi, G.,
Genco, R.J. and
De Nardin, E. Identification of linear
antigenic sites on the Porphyromonas
gingivalis 43 kDa fimbrillin subunit. Oral Microbiol. Immunol.,
10:146-150, 1995. Lala, A., Amano, A., Sojar,
H.T., Radel, S.J. and De Nardin., E.
Porphyromonas gingivalis
trypsin-like protease: a possible natural ligand for the neutrophil
formyl
peptide receptor. Biochem.
Biophys. Res. Comm., 199:1489-1496, 1994. De Nardin, E. Neutrophil
receptors:
N-formyl-l-methionyl-l-leucyl-l-phenylalanine and
Interleukin-8. In: Molecular
pathogenesis
of periodontal disease, Genco ed., ASM press, Washington DC, pp.
351-361, 1994. Radel, S.J., Genco, R.J. and De Nardin, E. Structural and functional characterization of
the human formyl peptide receptor ligand binding region.
Infect.
Immun., 62:1726-1732, 1994 Lala, A., Sharma, A., Sojar, H., Radel, S.,
Genco R. and De
Nardin, E. Recombinant Expression and
Partial Characterization of the Human Formyl Peptide Receptor. Biochim.
Biophys. Acta, 1178:302-306, 1993. Sorge, K.F., Potts, T.V. and De Nardin, E. Application of PCR to oral diagnostic. In:
PCR-Based Diagnostics, Ehrlich and
Greenberg editors. Blackwell Scientific
Publications, pp. 313-331, 1993. De Nardin, E., Radel, S.J., Lewis, N., Genco, R.J. and Hammarskjöld, M. Identification of a gene encoding for the
human formyl peptide receptor. Biochem.
Interl., 26:381-387, 1992. Radel, S., Genco, R.J. and De Nardin, E. Use of synthetic peptides for the functional
mapping of the human formyl peptide receptor. Biochem. Int., 25:745-753. Gillespie, J., De Nardin, E., Radel, S.J.
Kuracina, J.,
Smutko, J. and Zambon, J. Production of
an extracellular toxin by the oral pathogen Wolinella
recta. Microb. Pathogenesis, 12:69-77, 1992. De Nardin, E. and Genco, R.J.
Immunochemical characterization of the formyl
peptide receptor moieties on human neutrophils. Hybridoma, 10:49-63, 1991. De Nardin, E. Il
ruolo dei neutrofili nelle malattie parodontali. Il
Progresso Odontoiatrico, IV 3:15-20, 1991. De Nardin, E., De Luca, C., Levine, M.J. and
Genco, R.J.
Antibodies directed to the chemotactic factor receptors detect
differences
between chemotactically normal and defective neutrophils from LJP
patients. J. Periodontol., 61:609-617, 1990. |